Higher diversity of Brucella canis in Latin America, according to an MLVA_13 Bc analysis.

Autor: Boeri EJ; Instituto de Zoonosis Luis Pasteur, División Inmunología y Diagnóstico, Laboratorio Sección Serología y Diagnóstico, Ciudad Autónoma de Buenos Aires, Av. Díaz Velez 4821 CP 1405, Buenos Aires, Argentina. Electronic address: eduardoboeri@gmail.com., Ruybal P; Departamento de Microbiología, Universidad de Buenos Aires, Facultad de Medicina, Paraguay 2155, Buenos Aires, Argentina; CONICET - Universidad de Buenos Aires, Instituto de Investigaciones en Microbiología y Parasitología Médica (IMPaM), Paraguay 2157, Buenos Aires, Argentina., Dominguez ML; Instituto de Zoonosis Luis Pasteur, División Inmunología y Diagnóstico, Laboratorio Sección Serología y Diagnóstico, Ciudad Autónoma de Buenos Aires, Av. Díaz Velez 4821 CP 1405, Buenos Aires, Argentina., Fernandez NM; Instituto de Zoonosis Luis Pasteur, División Inmunología y Diagnóstico, Laboratorio Sección Serología y Diagnóstico, Ciudad Autónoma de Buenos Aires, Av. Díaz Velez 4821 CP 1405, Buenos Aires, Argentina., Becker P; Instituto de Zoonosis Luis Pasteur, División Inmunología y Diagnóstico, Laboratorio Sección Serología y Diagnóstico, Ciudad Autónoma de Buenos Aires, Av. Díaz Velez 4821 CP 1405, Buenos Aires, Argentina., Elena S; Laboratorio de Referencia de la OIE para Brucelosis, Dirección General de Laboratorio y Control Técnico (DiLab), Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA), Martínez, Buenos Aires 1640, Argentina., Escobar GI; Laboratorio de Brucelosis Laboratorio Nacional de Referencia INEI-ANLIS Dr. Carlos G. Malbrán. Av. Vélez Sarsfield 563, Ciudad Autónoma de Buenos Aires, Argentina., Ayala SM; Laboratorio de Brucelosis Laboratorio Nacional de Referencia INEI-ANLIS Dr. Carlos G. Malbrán. Av. Vélez Sarsfield 563, Ciudad Autónoma de Buenos Aires, Argentina., Hasan DB; Laboratorio de Brucelosis Laboratorio Nacional de Referencia INEI-ANLIS Dr. Carlos G. Malbrán. Av. Vélez Sarsfield 563, Ciudad Autónoma de Buenos Aires, Argentina., Trangoni MD; Laboratorio de Brucella, Campylobacter y Microbiota del rumen, Instituto de Biotecnología/Instituto de Agrobiotecnología y Biología Molecular (IB/IABIMO), UEDD INTA-CONICET, CICVyA, CNIA, INTA, Hurlingham, Argentina.
Jazyk: angličtina
Zdroj: Acta tropica [Acta Trop] 2023 Jul; Vol. 243, pp. 106914. Date of Electronic Publication: 2023 Apr 14.
DOI: 10.1016/j.actatropica.2023.106914
Abstrakt: Brucella canis is the main causative agent of canine brucellosis, which affects domestic and wild canids and leads to clinical signs and symptoms of the reproductive and locomotor systems. Owing to the scarce information on this pathogen, here we addressed the genetic diversity of the circulating strains of this species in Argentina by following an MVLA_13 Bc scheme. The analyzed sample set consisted of 101 strains of B. canis isolates collected between 2006 and 2020 from canines of the Autonomous City of Buenos Aires (CABA) and other regions of Argentina, as well as 235 isolates from North America. The analysis yielded 336 variants (Hunter-Gaston Diversity Index, HGDI equal to 1.0) showing high diversity on a global scale. The analysis of the six most variable markers also reveled high diversity and allowed further analysis regarding variant relationships. Although the diversity obtained using both schemes (all or the 6 most variable markers) was higher for the Latin American than for the North American strains, we cannot discard that this was due to biases in the sampling methodology or to the different health policies employed in these regions regarding the management of infected individuals. Altogether, the Argentine circulating strains are genetically diverse, but with no apparent geographical association. The markers used in the MLVA_13 Bc are variable and highly useful for the evaluation of outbreaks. Furthermore, the reduced panel of 6 markers (MLVA_6 Bc) proposed in this study is convenient for the study of B. canis strain diversity.
Competing Interests: Declaration of Competing Interest This research involved no conflicts of interest. The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: