Multilocus VNTR analysis-ompA typing of Chlamydia trachomatis isolates in Tehran, Iran.

Autor: Zarei A; Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran., Pourmand MR; Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: mpourmand@tums.ac.ir., Aminharati F; Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran., Zolfaghari P; Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran., Dehghan A; Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran., Emamie A; Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran., Movahedi S; Department of Obstetrics and Gynecology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Jazyk: angličtina
Zdroj: Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy [J Infect Chemother] 2023 Aug; Vol. 29 (8), pp. 759-763. Date of Electronic Publication: 2023 Apr 14.
DOI: 10.1016/j.jiac.2023.04.007
Abstrakt: Introduction: This study is the first to describe the genetic diversity of C. trachomatis strains derived from patients with signs and symptoms of genitourinary infections admitted to Tehran health centers and hospitals using the high-resolution genotyping method, multilocus variable-number tandem-repeat analysis with ompA sequencing (MLVA)-ompA.
Methods: One hundred and sixty-seven urogenital specimens were collected from October 2019 to July 2020. Specimens were inoculated to cell culture and examined for the presence of C. trachomatis isolates by microscopic valuation. Out of 167 samples, 19 (11.3%) viable C. trachomatis organisms were isolated in cell culture. Eighteen isolates were successfully genotyped by MLVA-ompA analysis.
Results: The most prevalent ompA genotypes were E, D, F and G, comprising 42%, 26.3% and 21% and 10.5% of isolates, respectively. Other genotypes were not detected from any of the samples. Out of the 18 fully genotyped isolates, 10 different MLVA-ompA genotypes were obtained. The most prevalent MLVA-ompA genotypes were 8.6.1-E (33.3%) and 8.5.2-D (16.6%). Genotype 8.6.1-E was common in both females and males.
Conclusions: Our results showed that MLVA-ompA analysis was more discriminatory than ompA typing alone and, therefore, a suitable complement to ompA. Using this method, dominant genotypes in the community and transmission patterns in sexual networks could be identified. The high diversity of C. trachomatis strains in Tehran may be due to the low level of public health and awareness, and future studies are needed.
Competing Interests: Declaration of competing interest The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
(Copyright © 2023 Japanese Society of Chemotherapy, Japanese Association for Infectious Diseases, and Japanese Society for Infection Prevention and Control. Published by Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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