Versatile microbial communities rapidly assimilate ammonium hydroxide-treated plastic waste.

Autor: Schaerer LG; Department of Biological Sciences, Michigan Technological University, Houghton, MI 49931, USA., Wood E; Department of Biological Sciences, Michigan Technological University, Houghton, MI 49931, USA., Aloba S; Department of Chemical Engineering, Michigan Technological University, Houghton, MI 49931, USA., Byrne E; Department of Biological Sciences, Michigan Technological University, Houghton, MI 49931, USA., Bashir MA; Department of Chemical Engineering, Michigan Technological University, Houghton, MI 49931, USA., Baruah K; Department of Chemical Engineering, Michigan Technological University, Houghton, MI 49931, USA., Schumann E; Department of Chemical Engineering, Michigan Technological University, Houghton, MI 49931, USA., Umlor L; Department of Chemical Engineering, Michigan Technological University, Houghton, MI 49931, USA., Wu R; Department of Chemical Engineering, Michigan Technological University, Houghton, MI 49931, USA., Lee H; Biological Processing Department, Idaho National Laboratory, Idaho Falls, ID 83415, USA., Orme CJ; Biological Processing Department, Idaho National Laboratory, Idaho Falls, ID 83415, USA., Wilson AD; Biological Processing Department, Idaho National Laboratory, Idaho Falls, ID 83415, USA., Lacey JA; Biological Processing Department, Idaho National Laboratory, Idaho Falls, ID 83415, USA., Ong RG; Department of Chemical Engineering, Michigan Technological University, Houghton, MI 49931, USA., Techtmann SM; Department of Biological Sciences, Michigan Technological University, Houghton, MI 49931, USA.
Jazyk: angličtina
Zdroj: Journal of industrial microbiology & biotechnology [J Ind Microbiol Biotechnol] 2023 Feb 17; Vol. 50 (1).
DOI: 10.1093/jimb/kuad008
Abstrakt: Waste plastic presently accumulates in landfills or the environment. While natural microbial metabolisms can degrade plastic polymers, biodegradation of plastic is very slow. This study demonstrates that chemical deconstruction of polyethylene terephthalate (PET) with ammonium hydroxide can replace the rate limiting step (depolymerization) and by producing plastic-derived terephthalic acid and terephthalic acid monoamide. The deconstructed PET (DCPET) is neutralized with phosphoric acid prior to bioprocessing, resulting in a product containing biologically accessible nitrogen and phosphorus from the process reactants. Three microbial consortia obtained from compost and sediment degraded DCPET in ultrapure water and scavenged river water without addition of nutrients. No statistically significant difference was observed in growth rate compared to communities grown on DCPET in minimal culture medium. The consortia were dominated by Rhodococcus spp., Hydrogenophaga spp., and many lower abundance genera. All taxa were related to species known to degrade aromatic compounds. Microbial consortia are known to confer flexibility in processing diverse substrates. To highlight this, we also demonstrate that two microbial consortia can grow on similarly deconstructed polyesters, polyamides, and polyurethanes in water instead of medium. Our findings suggest that microbial communities may enable flexible bioprocessing of mixed plastic wastes when coupled with chemical deconstruction.
(© The Author(s) 2023. Published by Oxford University Press on behalf of Society of Industrial Microbiology and Biotechnology.)
Databáze: MEDLINE
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