Stable endocytic structures navigate the complex pellicle of apicomplexan parasites.

Autor: Koreny L; Department of Biochemistry, University of Cambridge, Cambridge, CB2 1QW, UK., Mercado-Saavedra BN; Department of Biochemistry, University of Cambridge, Cambridge, CB2 1QW, UK., Klinger CM; Division of Infectious Diseases, Department of Medicine, University of Alberta, Edmonton, AB, T6G 2R3, Canada., Barylyuk K; Department of Biochemistry, University of Cambridge, Cambridge, CB2 1QW, UK., Butterworth S; Department of Biochemistry, University of Cambridge, Cambridge, CB2 1QW, UK., Hirst J; Cambridge Institute for Medical Research, University of Cambridge, Cambridge, CB2 1QW, UK., Rivera-Cuevas Y; Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, 48109, USA., Zaccai NR; Cambridge Institute for Medical Research, University of Cambridge, Cambridge, CB2 1QW, UK., Holzer VJC; Plant Development, Ludwig-Maximilians-University Munich, Planegg-Martinsried, 82152, Germany., Klingl A; Plant Development, Ludwig-Maximilians-University Munich, Planegg-Martinsried, 82152, Germany., Dacks JB; Division of Infectious Diseases, Department of Medicine, University of Alberta, Edmonton, AB, T6G 2R3, Canada.; Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice, 370 05, Czech Republic., Carruthers VB; Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, 48109, USA., Robinson MS; Cambridge Institute for Medical Research, University of Cambridge, Cambridge, CB2 1QW, UK., Gras S; Experimental Parasitology, Department for Veterinary Sciences, Ludwig-Maximilians-University Munich, Planegg-Martinsried, 82152, Germany. Simon.Gras@para.vetmed.uni-muenchen.de., Waller RF; Department of Biochemistry, University of Cambridge, Cambridge, CB2 1QW, UK. rfw26@cam.ac.uk.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2023 Apr 15; Vol. 14 (1), pp. 2167. Date of Electronic Publication: 2023 Apr 15.
DOI: 10.1038/s41467-023-37431-x
Abstrakt: Apicomplexan parasites have immense impacts on humanity, but their basic cellular processes are often poorly understood. Where endocytosis occurs in these cells, how conserved this process is with other eukaryotes, and what the functions of endocytosis are across this phylum are major unanswered questions. Using the apicomplexan model Toxoplasma, we identified the molecular composition and behavior of unusual, fixed endocytic structures. Here, stable complexes of endocytic proteins differ markedly from the dynamic assembly/disassembly of these machineries in other eukaryotes. We identify that these endocytic structures correspond to the 'micropore' that has been observed throughout the Apicomplexa. Moreover, conserved molecular adaptation of this structure is seen in apicomplexans including the kelch-domain protein K13 that is central to malarial drug-resistance. We determine that a dominant function of endocytosis in Toxoplasma is plasma membrane homeostasis, rather than parasite nutrition, and that these specialized endocytic structures originated early in infrakingdom Alveolata likely in response to the complex cell pellicle that defines this medically and ecologically important ancient eukaryotic lineage.
(© 2023. The Author(s).)
Databáze: MEDLINE
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