The EGCG and α-Mangosteen Stimulate SHED-IL10 and SHED-LL37 Metabolite Concentration.

Autor:	Yuliati Y; Doctoral Program, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.; Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia., Mahdani FY; Oral Medicine Study Program, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.; Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia., Margaretha SA; Bachelor of Dental Science Program. Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia., Yastuti WTP; Bachelor of Dental Science Program. Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia., Surboyo MDC; Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia., Aljunaid MA; Doctoral Program, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.; Department of Oral and Dental Medicine, Faculty of Medicine, Taiz University, Taiz, Yemen.; Faculty of Oral and Dental Medicine, Al-Saeed University, Taiz, Yemen., Qaid HR; Doctoral Program, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.; Faculty of Oral and Dental Medicine, Al-Saeed University, Taiz, Yemen., Ridwan RD; Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia., Diyatri I; Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
Jazyk:	angličtina
Zdroj:	European journal of dentistry [Eur J Dent] 2024 Feb; Vol. 18 (1), pp. 138-142. Date of Electronic Publication: 2023 Apr 14.
DOI:	10.1055/s-0043-1761460
Abstrakt:	Objective:  Stem cells of human exfoliated deciduous teeth (SHED) metabolites are secreted molecules from SHED, namely cytokines, chemokines, and growth factors. The metabolite can be used in various regenerative therapy based on cell-free immunomodulatory potential effects, like interleukin 10 (IL-10) and LL37. This molecule can stimulate with epigallocatechin gallate (EGCG) and α-mangosteen and has been proven to have anti-inflammatory and antibacterial effects. This study aimed to identify the effect of EGCG and α-mangosteen to SHED metabolite, called SHED-IL10 and SHED-LL37, from six passages to obtain the optimum stimulation and able to use as periodontitis regeneration treatment. Materials and Methods:  The six different passages of SHED were prepared in Dulbecco's modified Eagle medium and added with EGCG 80% (10 μM), EGCG 95% (10 μM), or α-mangosteen (10 μM). After a 24 hours incubation, each passage was measured with the metabolite concentration, SHED-IL10 and SHED-LL37, with human IL-10 and LL37 using enzyme-linked immunosorbent assay. Each different concentration was then analyzed statistically. Results:  The addition of EGCG 95% is able to stimulate the SHED-IL10 optimum concentration in passage 1 ( p  < 0.01). But, in the different conditions, the addition of EGCG 80%, EGCG 95%, and α-mangosteen was able to stimulate the SHED-LL37 optimum concentration in passage 2 ( p  < 0.001). Conclusion:  The addition of EGCG and α-mangosteen can stimulate the SHED-IL10 and SHED-LL37 concentrations. These two metabolites are promising as regenerative therapy through anti-inflammatory and antibacterial properties. Competing Interests: None declared. (The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/).)
Databáze:	MEDLINE
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