Proteome profiling highlights mechanisms underlying pigment and tocopherol accumulation in red and black rice seeds.

Autor: Gupta R; College of General Education, Kookmin University, Seoul, Republic of Korea., Min CW; Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang, Republic of Korea., Jung JY; Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang, Republic of Korea., Ham TH; Agricultural Science, Korea National Open University, Seoul, Republic of Korea., Jeon JS; Graduate School of Green-Bio Science and Crop Biotech Institute, Kyung Hee University, Yongin, Republic of Korea., Cho LH; Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang, Republic of Korea., Kwon SW; Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang, Republic of Korea., Kim ST; Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang, Republic of Korea.
Jazyk: angličtina
Zdroj: Proteomics [Proteomics] 2023 Jun; Vol. 23 (12), pp. e2300035. Date of Electronic Publication: 2023 Apr 14.
DOI: 10.1002/pmic.202300035
Abstrakt: Rice is a major component of the human diet and feeds more than 50 million people across the globe. We previously developed two pigmented rice cultivars, Super-hongmi (red seeds) and Super-jami (black seeds), that are highly rich in antioxidants and exhibit high levels of radical scavenging activities. However, the molecular mechanism underlying the accumulation of pigments and different antioxidants in these rice cultivars remains largely elusive. Here, we report the proteome profiles of mature Super-hongmi and Super-jami seeds, and compared them with the Hopum (white seeds) using a label-free quantitative proteomics approach. This approach led to the identification of 5127 rice seed proteins of which 1628 showed significant changes in the pigmented rice cultivar(s). The list of significantly modulated proteins included a phytoene desaturase (PDS3) which suggested accumulation of ζ-carotene in red seeds while the black seeds seem to accumulate more of anthocyanins because of the higher abundance of dihydroflavonol 4-reductase. Moreover, proteins associated with lignin and tocopherol biosynthesis were highly increased in both red and black cultivars. Taken together, these data report the seed proteome of three different colored rice seeds and identify novel components associated with pigment accumulation in rice.
(© 2023 Wiley-VCH GmbH.)
Databáze: MEDLINE
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