Previously uncharacterized rectangular bacterial structures in the dolphin mouth.

Autor: Dudek NK; Department of Medicine, Stanford University School of Medicine, Stanford, CA, 94305, USA.; Department of Ecology and Evolutionary Biology, University of California, Santa Cruz, Santa Cruz, CA, 95064, USA.; Quantori, Cambridge, MA, 02142, USA., Galaz-Montoya JG; Department of Bioengineering, Stanford University, Stanford, CA, 94305, USA., Shi H; Department of Bioengineering, Stanford University, Stanford, CA, 94305, USA.; Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, 94305, USA., Mayer M; Division of CryoEM and Bioimaging, SSRL, SLAC National Accelerator Laboratory, Menlo Park, CA, 94025, USA.; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, 02115, USA., Danita C; Department of Bioengineering, Stanford University, Stanford, CA, 94305, USA., Celis AI; Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, 94305, USA., Viehboeck T; Department of Functional and Evolutionary Ecology, Environmental Cell Biology Group, University of Vienna, Vienna, Austria.; Division of Microbial Ecology, Center for Microbiology and Environmental Systems Science, and Vienna Doctoral School of Ecology and Evolution, University of Vienna, Vienna, Austria., Wu GH; Department of Bioengineering, Stanford University, Stanford, CA, 94305, USA., Behr B; Department of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford, CA, 94305, USA., Bulgheresi S; Department of Functional and Evolutionary Ecology, Environmental Cell Biology Group, University of Vienna, Vienna, Austria., Huang KC; Department of Bioengineering, Stanford University, Stanford, CA, 94305, USA.; Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, 94305, USA.; Chan Zuckerberg Biohub, San Francisco, CA, 94158, USA., Chiu W; Department of Bioengineering, Stanford University, Stanford, CA, 94305, USA.; Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, 94305, USA.; Division of CryoEM and Bioimaging, SSRL, SLAC National Accelerator Laboratory, Menlo Park, CA, 94025, USA., Relman DA; Department of Medicine, Stanford University School of Medicine, Stanford, CA, 94305, USA. relman@stanford.edu.; Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, 94305, USA. relman@stanford.edu.; Chan Zuckerberg Biohub, San Francisco, CA, 94158, USA. relman@stanford.edu.; Infectious Diseases Section, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, 94304, USA. relman@stanford.edu.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2023 Apr 13; Vol. 14 (1), pp. 2098. Date of Electronic Publication: 2023 Apr 13.
DOI: 10.1038/s41467-023-37638-y
Abstrakt: Much remains to be explored regarding the diversity of uncultured, host-associated microbes. Here, we describe rectangular bacterial structures (RBSs) in the mouths of bottlenose dolphins. DNA staining revealed multiple paired bands within RBSs, suggesting the presence of cells dividing along the longitudinal axis. Cryogenic transmission electron microscopy and tomography showed parallel membrane-bound segments that are likely cells, encapsulated by an S-layer-like periodic surface covering. RBSs displayed unusual pilus-like appendages with bundles of threads splayed at the tips. We present multiple lines of evidence, including genomic DNA sequencing of micromanipulated RBSs, 16S rRNA gene sequencing, and fluorescence in situ hybridization, suggesting that RBSs are bacterial and distinct from the genera Simonsiella and Conchiformibius (family Neisseriaceae), with which they share similar morphology and division patterning. Our findings highlight the diversity of novel microbial forms and lifestyles that await characterization using tools complementary to genomics such as microscopy.
(© 2023. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply.)
Databáze: MEDLINE
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