Lysophosphatidic acid receptor LPA 1 trafficking and interaction with Rab proteins, as evidenced by Förster resonance energy transfer.

Autor: Martínez-Morales JC; Departamento de Biología Celular y Desarrollo, Ap. Postal 70-600, Ciudad de México, 04510, Mexico., González-Ruiz KD; Departamento de Biología Celular y Desarrollo, Ap. Postal 70-600, Ciudad de México, 04510, Mexico., Romero-Ávila MT; Departamento de Biología Celular y Desarrollo, Ap. Postal 70-600, Ciudad de México, 04510, Mexico., Rincón-Heredia R; Unidad de Imagenología, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad Universitaria, Ap. Postal 70-600, Ciudad de México, 04510, Mexico., Reyes-Cruz G; Departamento de Biología Celular, Centro de Investigación y Estudios Avanzados-Instituto Politécnico Nacional, Colonia San Pedro Zacatenco, Ciudad de México, 07360, Mexico., García-Sáinz JA; Departamento de Biología Celular y Desarrollo, Ap. Postal 70-600, Ciudad de México, 04510, Mexico. Electronic address: agarcia@ifc.unam.mx.
Jazyk: angličtina
Zdroj: Molecular and cellular endocrinology [Mol Cell Endocrinol] 2023 Jun 15; Vol. 570, pp. 111930. Date of Electronic Publication: 2023 Apr 11.
DOI: 10.1016/j.mce.2023.111930
Abstrakt: LPA 1 internalization to endosomes was studied employing Förster Resonance Energy Transfer (FRET) in cells coexpressing the mCherry-lysophosphatidic acid LPA 1 receptors and distinct eGFP-tagged Rab proteins. Lysophosphatidic acid (LPA)-induced internalization was rapid and decreased afterward: phorbol myristate acetate (PMA) action was slower and sustained. LPA stimulated LPA 1 -Rab5 interaction rapidly but transiently, whereas PMA action was rapid but sustained. Expression of a Rab5 dominant-negative mutant blocked LPA 1 -Rab5 interaction and receptor internalization. LPA-induced LPA 1 -Rab9 interaction was only observed at 60 min, and LPA 1 -Rab7 interaction after 5 min with LPA and after 60 min with PMA. LPA triggered immediate but transient rapid recycling (i.e., LPA 1 -Rab4 interaction), whereas PMA action was slower but sustained. Agonist-induced slow recycling (LPA 1 -Rab11 interaction) increased at 15 min and remained at this level, whereas PMA action showed early and late peaks. Our results indicate that LPA 1 receptor internalization varies with the stimuli.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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