Visualizing dynamic three-dimensional changes of human reticular dermal collagen under mechanical strain.

Autor:	van Haasterecht L; LaserLab Amsterdam, Department of Physics and Astronomy, Faculty of Sciences Vrije Universiteit, Amsterdam, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands.; Amsterdam UMC, Vrije Universiteit, Department of Plastic, Reconstructive and Hand Surgery, Amsterdam Movement Sciences, PO Box 7057, 1007 MB Amsterdam, The Netherlands.; Burn Center, Red Cross Hospital, PO Box 1074, 1940 EB Beverwijk, The Netherlands., Zhou M; LaserLab Amsterdam, Department of Physics and Astronomy, Faculty of Sciences Vrije Universiteit, Amsterdam, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands., Ma Y; LaserLab Amsterdam, Department of Physics and Astronomy, Faculty of Sciences Vrije Universiteit, Amsterdam, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands., Bartolini L; LaserLab Amsterdam, Department of Physics and Astronomy, Faculty of Sciences Vrije Universiteit, Amsterdam, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands., Van Mourik F; LaserLab Amsterdam, Department of Physics and Astronomy, Faculty of Sciences Vrije Universiteit, Amsterdam, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands., Van Zuijlen PPM; Amsterdam UMC, Vrije Universiteit, Department of Plastic, Reconstructive and Hand Surgery, Amsterdam Movement Sciences, PO Box 7057, 1007 MB Amsterdam, The Netherlands.; Burn Center, Red Cross Hospital, PO Box 1074, 1940 EB Beverwijk, The Netherlands., Groot ML; LaserLab Amsterdam, Department of Physics and Astronomy, Faculty of Sciences Vrije Universiteit, Amsterdam, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands.
Jazyk:	angličtina
Zdroj:	Biomedical physics & engineering express [Biomed Phys Eng Express] 2023 Apr 25; Vol. 9 (3). Date of Electronic Publication: 2023 Apr 25.
DOI:	10.1088/2057-1976/accc8e
Abstrakt:	In clinical practice, plastic surgeons are often faced with large skin defects that are difficult to close primarily. Management of large skin wounds e.g. burns or traumatic lacerations requires knowledge of skin biomechanic properties. Research into skin microstructural adaptation to mechanical deformation has only been performed using static regimes due to technical limitations. Here, we combine uniaxial stretch tests with fast second harmonic generation imaging and we apply this for the first time to investigate dynamic collagen rearrangement in reticular human dermis. Ex vivo human skin from the abdomen and upper thigh was simultaneously uniaxially stretched while either periodically visualizing 3D reorganization, or visualizing 2D changes in real time. We determined collagen alignment via orientation indices and found pronounced variability across samples. Comparing mean orientation indices at the different stages of the stress strain curves (toe, heel, linear) showed a significant increase in collagen alignment during the linear part of the mechanical response. We conclude that fast SHG imaging during uni-axial extension is a promising research tool for future studies on skin biomechanic properties. (Creative Commons Attribution license.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu