A DEAD-box RNA helicase mediates meiotic silencing by unpaired DNA.

Autor:	Sy VT; Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA., Boone EC; Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA., Xiao H; Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA., Vierling MM; Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA., Schmitz SF; Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA., Ung Q; Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA., Trawick SS; Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA., Hammond TM; School of Biological Sciences, Illinois State University, Normal, IL 61790, USA., Shiu PKT; Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA.
Jazyk:	angličtina
Zdroj:	G3 (Bethesda, Md.) [G3 (Bethesda)] 2023 Aug 09; Vol. 13 (8).
DOI:	10.1093/g3journal/jkad083
Abstrakt:	During the sexual phase of Neurospora crassa, unpaired genes are subject to a silencing mechanism known as meiotic silencing by unpaired DNA (MSUD). MSUD targets the transcripts of an unpaired gene and utilizes typical RNA interference factors for its process. Using a reverse genetic screen, we have identified a meiotic silencing gene called sad-9, which encodes a DEAD-box RNA helicase. While not essential for vegetative growth, SAD-9 plays a crucial role in both sexual development and MSUD. Our results suggest that SAD-9, with the help of the SAD-2 scaffold protein, recruits the SMS-2 Argonaute to the perinuclear region, the center of MSUD activity. Competing Interests: Conflicts of interest None declared. (© The Author(s) 2023. Published by Oxford University Press on behalf of The Genetics Society of America.)
Databáze:	MEDLINE
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