Isolated Peptide from Spider Venom Modulates Dendritic Cells In Vitro: A Possible Application in Oncoimmunotherapy for Glioblastoma.

Autor: de Mato FC; Faculdade de Ciências Farmacêuticas, Universidade Estadual de Campinas (UNICAMP), Campinas 13083-871, SP, Brazil.; Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, UNICAMP, Campinas 13083-862, SP, Brazil., Barreto N; Faculdade de Ciências Farmacêuticas, Universidade Estadual de Campinas (UNICAMP), Campinas 13083-871, SP, Brazil.; Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, UNICAMP, Campinas 13083-862, SP, Brazil., Cordeiro G; Faculdade de Ciências Farmacêuticas, Universidade Estadual de Campinas (UNICAMP), Campinas 13083-871, SP, Brazil.; Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, UNICAMP, Campinas 13083-862, SP, Brazil., Munhoz J; Department of Agricultural, Food and Nutritional Sciences (AFNS), University of Alberta, Edmonton, AB T6G 2R3, Canada., Bonfanti AP; Faculdade de Ciências Farmacêuticas, Universidade Estadual de Campinas (UNICAMP), Campinas 13083-871, SP, Brazil.; Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, UNICAMP, Campinas 13083-862, SP, Brazil., da Rocha-E-Silva TAA; Department of Physiological Sciences, Faculdade Israelita de Ciências da Saúde Albert Einstein, São Paulo 05653-120, SP, Brazil., Sutti R; Valer Laboratórios Eireli, São Paulo 13347-633, SP, Brazil., Cruz PBM; Department of Physiological Sciences, Faculdade Israelita de Ciências da Saúde Albert Einstein, São Paulo 05653-120, SP, Brazil., Sanches LR; Department of Physiological Sciences, Faculdade Israelita de Ciências da Saúde Albert Einstein, São Paulo 05653-120, SP, Brazil., Bombeiro AL; Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, UNICAMP, Campinas 13083-862, SP, Brazil., Chalbatani GM; Department of Immunology, Mayo Clinic, Scottsdale, AZ 85259, USA., Verinaud L; Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, UNICAMP, Campinas 13083-862, SP, Brazil., Rapôso C; Faculdade de Ciências Farmacêuticas, Universidade Estadual de Campinas (UNICAMP), Campinas 13083-871, SP, Brazil.; Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, UNICAMP, Campinas 13083-862, SP, Brazil.
Jazyk: angličtina
Zdroj: Cells [Cells] 2023 Mar 27; Vol. 12 (7). Date of Electronic Publication: 2023 Mar 27.
DOI: 10.3390/cells12071023
Abstrakt: Dendritic cells (DCs) vaccine is a potential tool for oncoimmunotherapy. However, it is known that this therapeutic strategy has failed in solid tumors, making the development of immunoadjuvants highly relevant. Recently, we demonstrated that Phoneutria nigriventer spider venom (PnV) components are cytotoxic to glioblastoma (GB) and activate macrophages for an antitumor profile. However, the effects of these molecules on the adaptive immune response have not yet been evaluated. This work aimed to test PnV and its purified fractions in DCs in vitro. For this purpose, bone marrow precursors were collected from male C57BL6 mice, differentiated into DCs and treated with venom or PnV-isolated fractions (F1-molecules < 3 kDa, F2-3 to 10 kDa and F3->10 kDa), with or without costimulation with human GB lysate. The results showed that mainly F1 was able to activate DCs, increasing the activation-dependent surface marker (CD86) and cytokine release (IL-1β, TNF-α), in addition to inducing a typical morphology of mature DCs. From the F1 purification, a molecule named LW9 was the most effective, and mass spectrometry showed it to be a peptide. The present findings suggest that this molecule could be an immunoadjuvant with possible application in DC vaccines for the treatment of GB.
Databáze: MEDLINE
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