Bowel Preparation for Colonoscopy Changes Serum Composition as Detected by Thermal Liquid Biopsy and Fluorescence Spectroscopy.

Autor: Hermoso-Durán S; Institute of Biocomputation and Physics of Complex Systems (BIFI), Joint Unit GBsC-CSIC-BIFI, University of Zaragoza, 50018 Zaragoza, Spain.; Aragón Health Research Institute (IIS Aragón), 50009 Zaragoza, Spain.; Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), 28029 Madrid, Spain., Domper-Arnal MJ; Aragón Health Research Institute (IIS Aragón), 50009 Zaragoza, Spain.; Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), 28029 Madrid, Spain.; Department of Gastroenterology, Lozano Blesa Clinic University Hospital, 50009 Zaragoza, Spain., Roncales P; Aragón Health Research Institute (IIS Aragón), 50009 Zaragoza, Spain.; Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), 28029 Madrid, Spain.; Department of Gastroenterology, Lozano Blesa Clinic University Hospital, 50009 Zaragoza, Spain., Vega S; Institute of Biocomputation and Physics of Complex Systems (BIFI), Joint Unit GBsC-CSIC-BIFI, University of Zaragoza, 50018 Zaragoza, Spain., Sanchez-Gracia O; Department of Electronic Engineering and Communications, University of Zaragoza, 50009 Zaragoza, Spain.; SOTER BioAnalytics, Enrique Val, 50011 Zaragoza, Spain., Ojeda JL; Department of Statistical Methods, University of Zaragoza, 50009 Zaragoza, Spain., Lanas Á; Aragón Health Research Institute (IIS Aragón), 50009 Zaragoza, Spain.; Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), 28029 Madrid, Spain.; Department of Gastroenterology, Lozano Blesa Clinic University Hospital, 50009 Zaragoza, Spain.; Department of Medicine, Psychiatry and Dermatology, University of Zaragoza, 50009 Zaragoza, Spain., Velazquez-Campoy A; Institute of Biocomputation and Physics of Complex Systems (BIFI), Joint Unit GBsC-CSIC-BIFI, University of Zaragoza, 50018 Zaragoza, Spain.; Aragón Health Research Institute (IIS Aragón), 50009 Zaragoza, Spain.; Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), 28029 Madrid, Spain.; Department of Biochemistry and Molecular and Cell Biology, University of Zaragoza, 50009 Zaragoza, Spain., Abian O; Institute of Biocomputation and Physics of Complex Systems (BIFI), Joint Unit GBsC-CSIC-BIFI, University of Zaragoza, 50018 Zaragoza, Spain.; Aragón Health Research Institute (IIS Aragón), 50009 Zaragoza, Spain.; Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), 28029 Madrid, Spain.; Department of Biochemistry and Molecular and Cell Biology, University of Zaragoza, 50009 Zaragoza, Spain.
Jazyk: angličtina
Zdroj: Cancers [Cancers (Basel)] 2023 Mar 24; Vol. 15 (7). Date of Electronic Publication: 2023 Mar 24.
DOI: 10.3390/cancers15071952
Abstrakt: (1) Background: About 50% of prescribed colonoscopies report no pathological findings. A secondary screening test after fecal immunochemical test positivity (FIT+) would be required. Considering thermal liquid biopsy (TLB) as a potential secondary test, the aim of this work was to study possible interferences of colonoscopy bowel preparation on TLB outcome on a retrospective study; (2) Methods: Three groups were studied: 1/514 FIT(+) patients enrolled in a colorectal screening program (CN and CP with normal and pathological colonoscopy, respectively), with blood samples obtained just before colonoscopy and after bowel preparation; 2/55 patients from the CN group with blood sample redrawn after only standard 8-10 h fasting and no bowel preparation (CNR); and 3/55 blood donors from the biobank considered as a healthy control group; (3) Results: The results showed that from the 514 patients undergoing colonoscopy, 247 had CN and 267 had CP. TLB parameters in these two groups were similar but different from those of the blood donors. The resampled patients (with normal colonoscopy and no bowel preparation) had similar TLB parameters to those of the blood donors. TLB parameters together with fluorescence spectra and other serum indicators (albumin and C-reactive protein) confirmed the statistically significant differences between normal colonoscopy patients with and without bowel preparation; (4) Conclusions: Bowel preparation seemed to alter serum protein levels and altered TLB parameters (different from a healthy subject). The diagnostic capability of other liquid-biopsy-based methods might also be compromised. Blood extraction after bowel preparation for colonoscopy should be avoided.
Databáze: MEDLINE
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