Rational design of a survey protocol for avocado sunblotch viroid in commercial orchards to demonstrate pest freedom.

Autor: Bonnéry DB; Department of Plant Sciences, University of Cambridge, Cambridge, United Kingdom., Pretorius LS; Centre for Horticultural Science, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, QLD, Australia., Jooste AEC; Agricultural Research Council-Tropical and Subtropical Crops, Mbombela, South Africa., Geering ADW; Centre for Horticultural Science, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, QLD, Australia., Gilligan CA; Department of Plant Sciences, University of Cambridge, Cambridge, United Kingdom.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2023 Apr 11; Vol. 18 (4), pp. e0277725. Date of Electronic Publication: 2023 Apr 11 (Print Publication: 2023).
DOI: 10.1371/journal.pone.0277725
Abstrakt: Avocado sunblotch viroid (ASBVd) is a subcellular pathogen of avocado that reduces yield from a tree, diminishes the appearance of the fruit by causing unsightly scarring and impedes trade because of quarantine conditions that are imposed to prevent spread of the pathogen via seed-borne inoculum. For countries where ASBVd is officially reported, permission to export fruit to another country may only be granted if an orchard can be demonstrated to be a pest free production site. The survey requirements to demonstrate pest freedom are usually defined in export protocols that have been mutually agreed upon by the trading partners. In this paper, we introduce a flexible statistical protocol for use in optimizing sampling strategies to establish pest free status from ASBVd in avocado orchards. The protocol, which is supported by an interactive app, integrates statistical considerations of multistage sampling of trees in orchards with a RT-qPCR assay allowing for detection of infection in pooled samples of leaves taken from multiple trees. While this study was motivated by a need to design a survey protocol for ASBVd, the theoretical framework and the accompanying app have broader applicability to a range of plant pathogens in which hierarchical sampling of a target population is coupled with pooling of material prior to diagnosis.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2023 Bonnéry et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje