| Autor: |
Sadeghi Z; Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Evin, Tehran 1983969411, Iran.; Dipartimento di Farmacia, Università degli Studi di Salerno, via Giovanni Paolo II n. 132, 84084 Fisciano, Salerno, Italy., Cerulli A; Dipartimento di Farmacia, Università degli Studi di Salerno, via Giovanni Paolo II n. 132, 84084 Fisciano, Salerno, Italy., Marzocco S; Dipartimento di Farmacia, Università degli Studi di Salerno, via Giovanni Paolo II n. 132, 84084 Fisciano, Salerno, Italy., Moridi Farimani M; Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Evin, Tehran 1983969411, Iran., Masullo M; Dipartimento di Farmacia, Università degli Studi di Salerno, via Giovanni Paolo II n. 132, 84084 Fisciano, Salerno, Italy., Piacente S; Dipartimento di Farmacia, Università degli Studi di Salerno, via Giovanni Paolo II n. 132, 84084 Fisciano, Salerno, Italy. |
| Abstrakt: |
Perovskia artemisioides is a perennial and aromatic plant widely distributed in the Baluchestan region of Iran. Phytochemical analysis of a n -hexane extract of P. artemisioides roots, guided by an analytical approach based on LC-ESI/LTQOrbitrap/MS/MS, yielded six previously undescribed diterpenoid compounds ( 2 , 9 - 11 , 16 , and 20 ), and 19 known diterpenoids, for which the structures were elucidated by 1D and 2D NMR experiments. Some of the isolated compounds showed significant anti-inflammatory activity using J774A.1 macrophage cells stimulated with Escherichia coli lipopolysaccharide. In particular, compounds 6 , 8 , 17 , 18 , 20 , and 22 significantly inhibited the release of nitric oxide and the expression of related pro-inflammatory enzymes, such as inducible nitric oxide synthase and cycloxygenase-2. Moreover, two compounds that showed the highest activity in reducing nitric oxide release ( 6 and 18 ) were tested to evaluate their effects on nitrotyrosine formation and reactive oxygen species release. Both compounds inhibited ROS release and, in particular, compound 6 also inhibited nitrotyrosine formation at all tested concentrations, thus indicating a significant antioxidant potential. |
