Dual-targeting surface-enhancement Raman scattering tags based on silver nanocubes for early diagnosis of pheochromocytoma.

Autor: Meng Y; Department of Chemistry, School of Science, China Pharmaceutical University, Nanjing, 211198, Jiangsu, PR China; Cell and Biomolecule Recognition Research Center, School of Science, China Pharmaceutical University, Nanjing, 211198, Jiangsu, PR China., Zhang Y; Department of Chemistry, School of Science, China Pharmaceutical University, Nanjing, 211198, Jiangsu, PR China; Cell and Biomolecule Recognition Research Center, School of Science, China Pharmaceutical University, Nanjing, 211198, Jiangsu, PR China., Liu J; Department of Chemistry, School of Science, China Pharmaceutical University, Nanjing, 211198, Jiangsu, PR China; Cell and Biomolecule Recognition Research Center, School of Science, China Pharmaceutical University, Nanjing, 211198, Jiangsu, PR China., Zhao L; Department of Chemistry, School of Science, China Pharmaceutical University, Nanjing, 211198, Jiangsu, PR China; Cell and Biomolecule Recognition Research Center, School of Science, China Pharmaceutical University, Nanjing, 211198, Jiangsu, PR China., Ren Q; Tibetan Medicine Research Institute, University of Tibetan Medicine, Tibet, 850000, PR China., Wang F; Department of Chemistry, School of Science, China Pharmaceutical University, Nanjing, 211198, Jiangsu, PR China; Cell and Biomolecule Recognition Research Center, School of Science, China Pharmaceutical University, Nanjing, 211198, Jiangsu, PR China. Electronic address: feiwang@cpu.edu.cn., Li C; Department of Chemistry, School of Science, China Pharmaceutical University, Nanjing, 211198, Jiangsu, PR China; Cell and Biomolecule Recognition Research Center, School of Science, China Pharmaceutical University, Nanjing, 211198, Jiangsu, PR China. Electronic address: licl@cpu.edu.cn.
Jazyk: angličtina
Zdroj: Analytica chimica acta [Anal Chim Acta] 2023 May 22; Vol. 1256, pp. 341148. Date of Electronic Publication: 2023 Mar 27.
DOI: 10.1016/j.aca.2023.341148
Abstrakt: Pheochromocytoma (PCC), a rare tumor, often develops distant metastases after diagnosis, delaying early intervention treatment. In order to overcome the limitations of traditional diagnostic methods, dual-targeting Surface-Enhancement Raman Scattering (SERS) cytosensor was developed to identify and detect PCC-CTCs from peripheral blood. Meta-iodobenzylguanidine (MIBG) and octreotide-2,2',2″,2'''- (1,4,7,10 -tetraazacyclododecane-1,4,7,10-tetrayl) tetraacetic acid (DOTA) functionalized magnetic Fe 3 O 4 and Ag-DTNB were prepared as capture probe and signal probe for SERS signal export, respectively. Ag nanocubes (AgNCs) as Raman active substrate offer an enhanced electromagnetic field, which could effectively enhance the signal intensity of DTNB and potentially realize trace analyte detection. The obtained SERS fingerprint spectroscopy possessed the characteristic of high sensitivity and resolution in the concentration range from 3.0-3.0 × 10 6  cells mL -1 , with a detection limit of 1 cell mL -1 , which laterally compensated the deficiency of scarce CTCs in peripheral blood. This work provided new insight into PCC-CTCs accurate detection.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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