Chimeric flavivirus causes vascular leakage and bone marrow suppression in a mouse model.

Autor: Kurosu T; Research Institute for Microbial Diseases (RIMD), Osaka University, Suita, Osaka, 565-0871, Japan; Department of Virology I, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama-shi, Tokyo, 208-0011, Japan. Electronic address: kurosu@niid.go.jp., Hanabara K; Research Institute for Microbial Diseases (RIMD), Osaka University, Suita, Osaka, 565-0871, Japan., Asai A; Research Institute for Microbial Diseases (RIMD), Osaka University, Suita, Osaka, 565-0871, Japan., Pambudi S; Research Institute for Microbial Diseases (RIMD), Osaka University, Suita, Osaka, 565-0871, Japan., Phanthanawiboon S; Research Institute for Microbial Diseases (RIMD), Osaka University, Suita, Osaka, 565-0871, Japan., Omokoko MD; Research Institute for Microbial Diseases (RIMD), Osaka University, Suita, Osaka, 565-0871, Japan., Sakai Y; Department of Pathology, National Institute of Infectious Diseases, Tokyo, 208-0011, Japan., Suzuki T; Department of Pathology, National Institute of Infectious Diseases, Tokyo, 208-0011, Japan., Ikuta K; Research Institute for Microbial Diseases (RIMD), Osaka University, Suita, Osaka, 565-0871, Japan.
Jazyk: angličtina
Zdroj: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2023 Jun 04; Vol. 659, pp. 54-61. Date of Electronic Publication: 2023 Apr 05.
DOI: 10.1016/j.bbrc.2023.04.003
Abstrakt: Previously, we demonstrated the utility of a recombinant chimeric flavivirus (DV2ChimV), which carries the premembrane (prM) and envelope (E) genes of a type 2 DENV clinical (Thai) isolate on a backbone of Japanese encephalitis virus, for evaluating the protective efficacy of antidengue envelope antibodies both in vitro and in vivo. Here, to assess the potential use of this model for pathological studies, we aimed to characterize interferon-α/β-γ-receptor double-knockout mice (IFN-α/β/γR dKO mice) infected with DV2ChimV. Vascular leakage and bone marrow suppression are unique features of severe dengue. In the current model, DV2ChimV caused vascular leakage in the liver and intestine at the moribund stage. High levels of virus were detected in the bone marrow, and strong bone marrow suppression (i.e., disappearance of megakaryocytes and erythroblastic islets) was observed. These observations suggest that the DV2ChimV-infected mouse model mimics the vascular leakage and bone marrow suppression observed in human cases.
Competing Interests: Declaration of competing interest The authors declare no conflicts of interest.
(Copyright © 2023 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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