| Autor: |
Rguez S; Laboratory of Medicinal and Aromatic Plants, Centre of Biotechnology of Borj Cedria, BP. 901, Hammam-Lif 2050, Tunisia., Hamrouni Sellami I; Laboratory of Medicinal and Aromatic Plants, Centre of Biotechnology of Borj Cedria, BP. 901, Hammam-Lif 2050, Tunisia., Abid G; Laboratory of Legumes and Sustainable Agro-systems, Centre of Biotechnology of Borj Cedria, Tunisia., Papetti A; Nutraceutical & Food Chemical-Toxicological Analysis Laboratory, Department of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, Italy., Hammami M; Laboratory of Medicinal and Aromatic Plants, Centre of Biotechnology of Borj Cedria, BP. 901, Hammam-Lif 2050, Tunisia., Souissi F; Laboratory of Legumes and Sustainable Agro-systems, Centre of Biotechnology of Borj Cedria, Tunisia., Chaouachi M; Laboratory of Bioactive Substances, Centre of Biotechnology of Borj Cedria, Tunisia., Muhovski Y; Department of Life Sciences, Walloon Agricultural Research Centre, Gembloux, Belgium., Msaada K; Laboratory of Medicinal and Aromatic Plants, Centre of Biotechnology of Borj Cedria, BP. 901, Hammam-Lif 2050, Tunisia., Djébali N; Laboratory of Bioactive Substances, Centre of Biotechnology of Borj Cedria, Tunisia. |
| Abstrakt: |
Tetraclinis articulata essential oil proved to be effective in controlling tomato grey mould, so we would investigate its effect on some tomato defense mechanisms. The pretreatment of Botrytis cinerea infected tomato plants with TAEO emulsion enhanced the activity of antioxidant enzymes activity SOD, CAT, APX, and GPX, and total polyphenols content and it decreased IC 50 of free radical-scavenging activity and H 2 O 2 content. Results showed amelioration in antioxidant status in TAEO emulsion treated and B. cinerea infected plants indicating that treatment decreased infection in tomato plants. The qRT-PCR analysis of defense genes expression Chitinase SlChi, transcription factors SlWRKY and SlAP2/ERF, Lipoxygenase SlLOX, and Thioredoxin SlTRX showed that they were up-regulated as early as 12 hpi sustained with a second increase at 48 hpi in TAEO emulsion pretreated and infected plants. These results suggest the potential use of TAEO emulsion as natural product to induce tomato antioxidant status and activate defense genes. |
