Sequence diversity of hepatitis D virus in Mongolia.

Autor: Magvan B; Department of Microbiology and Infection Prevention and Control, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia., Kloeble AA; Institute of Virology, University Hospital Düsseldorf, Düsseldorf, Germany., Ptok J; Institute of Virology, University Hospital Düsseldorf, Düsseldorf, Germany., Hoffmann D; Bioinformatics and Computational Biophysics, Faculty of Biology, University of Duisburg-Essen, Essen, Germany., Habermann D; Bioinformatics and Computational Biophysics, Faculty of Biology, University of Duisburg-Essen, Essen, Germany., Gantumur A; Department of Microbiology and Infection Prevention and Control, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia., Paluschinski M; Institute of Virology, University Hospital Düsseldorf, Düsseldorf, Germany., Enebish G; Department of Microbiology and Infection Prevention and Control, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia., Balz V; Institute for Transplant Diagnostics and Cell Therapeutics, University Hospital Dusseldorf, Dusseldorf, Germany., Fischer JC; Institute for Transplant Diagnostics and Cell Therapeutics, University Hospital Dusseldorf, Dusseldorf, Germany., Chimeddorj B; Department of Microbiology and Infection Prevention and Control, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia.; Institute of Biomedical Sciences, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia., Walker A; Institute of Virology, University Hospital Düsseldorf, Düsseldorf, Germany., Timm J; Institute of Virology, University Hospital Düsseldorf, Düsseldorf, Germany.
Jazyk: angličtina
Zdroj: Frontiers in medicine [Front Med (Lausanne)] 2023 Mar 13; Vol. 10, pp. 1108543. Date of Electronic Publication: 2023 Mar 13 (Print Publication: 2023).
DOI: 10.3389/fmed.2023.1108543
Abstrakt: Introduction: The Hepatitis Delta Virus (HDV) is a defective, single-stranded RNA virusoid encoding for a single protein, the Hepatitis Delta Antigen (HDAg), which requires the hepatitis B virus (HBV) envelope protein (HBsAg) for its transmission. Currently, hepatitis D is the most aggressive form of viral hepatitis and treatment options are limited. Worldwide 12 million people are chronically infected with HDV being at high risk for progression to cirrhosis and development of liver cancer.
Objectives: Although it is well established that Mongolia is the country with the highest prevalence of HDV infections, the information on the molecular epidemiology and factors contributing to HDV sequence diversity are largely unclear. The aim of the study was to characterize the sequence diversity of HDV in rural areas from Mongolia and to determine the extent of HLA class I-associated selection pressure.
Patients and Methods: From the HepMongolia cohort from rural areas in Mongolia, 451 HBsAg-positive individuals were selected and anti-HDV, HDV-RNA and the sequence of the large HDAg was determined. For all individuals the HLA class I locus was genotyped. Residues under selection pressure in the presence of individual HLA class I types were identified with the recently published analysis tool HAMdetector.
Results: Of 431 HBsAg positive patients, 281 were anti-HDV positive (65%), and HDV-RNA could be detected in 207 of 281 (74%) of patients. The complete large HDAg was successfully sequenced from 131 samples. Phylogenetic analysis revealed that all Mongolian HDV isolates belong to genotype 1, however, they separate into several different clusters without clear regional association. In turn, from phylogeny there is strong evidence for recent local transmission events. Importantly, we found multiple residues with strong support for HLA class I-associated selection pressure consistent with a functional CD8 + T cell response directed against HDV.
Conclusion: HDV isolates from Mongolia are highly diverse. The molecular epidemiology suggests circulation of multiple subtypes and provides evidence for ongoing recent transmissions.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2023 Magvan, Kloeble, Ptok, Hoffmann, Habermann, Gantumur, Paluschinski, Enebish, Balz, Fischer, Chimeddorj, Walker and Timm.)
Databáze: MEDLINE