Methacrylated human recombinant collagen peptide as a hydrogel for manipulating and monitoring stiffness-related cardiac cell behavior.

Autor:	Mostert D; Department of Biomedical Engineering, Eindhoven University of Technology, 5600 MB Eindhoven, the Netherlands.; Institute for Complex Molecular Systems (ICMS), 5600 MB Eindhoven, the Netherlands., Jorba I; Department of Biomedical Engineering, Eindhoven University of Technology, 5600 MB Eindhoven, the Netherlands.; Institute for Complex Molecular Systems (ICMS), 5600 MB Eindhoven, the Netherlands., Groenen BGW; Department of Biomedical Engineering, Eindhoven University of Technology, 5600 MB Eindhoven, the Netherlands., Passier R; Department of Applied Stem Cell Technologies, University of Twente, 7522 NB Enschede, the Netherlands.; Department of Anatomy and Embryology, Leiden University Medical Centre, 2333 ZA Leiden, the Netherlands., Goumans MTH; Department of Cell and Chemical Biology and Center for Biomedical Genetics, Leiden University Medical Centre, 2333 ZA Leiden, the Netherlands., van Boxtel HA; Fujifilm Manufacturing Europe B.V., 5047 TK Tilburg, the Netherlands., Kurniawan NA; Department of Biomedical Engineering, Eindhoven University of Technology, 5600 MB Eindhoven, the Netherlands.; Institute for Complex Molecular Systems (ICMS), 5600 MB Eindhoven, the Netherlands., Bouten CVC; Department of Biomedical Engineering, Eindhoven University of Technology, 5600 MB Eindhoven, the Netherlands.; Institute for Complex Molecular Systems (ICMS), 5600 MB Eindhoven, the Netherlands., Klouda L; Department of Biomedical Engineering, Eindhoven University of Technology, 5600 MB Eindhoven, the Netherlands.; Department of Engineering, Rangos School of Health Sciences, Duquesne University, Pittsburgh, PA 15282, USA.
Jazyk:	angličtina
Zdroj:	IScience [iScience] 2023 Mar 16; Vol. 26 (4), pp. 106423. Date of Electronic Publication: 2023 Mar 16 (Print Publication: 2023).
DOI:	10.1016/j.isci.2023.106423
Abstrakt:	Environmental stiffness is a crucial determinant of cell function. There is a long-standing quest for reproducible and (human matrix) bio-mimicking biomaterials with controllable mechanical properties to unravel the relationship between stiffness and cell behavior. Here, we evaluate methacrylated human recombinant collagen peptide (RCPhC1-MA) hydrogels as a matrix to control 3D microenvironmental stiffness and monitor cardiac cell response. We show that RCPhC1-MA can form hydrogels with reproducible stiffness in the range of human developmental and adult myocardium. Cardiomyocytes (hPSC-CMs) and cardiac fibroblasts (cFBs) remain viable for up to 14 days inside RCPhC1-MA hydrogels while the effect of hydrogel stiffness on extracellular matrix production and hPSC-CM contractility can be monitored in real-time. Interestingly, whereas the beating behavior of the hPSC-CM monocultures is affected by environmental stiffness, this effect ceases when cFBs are present. Together, we demonstrate RCPhC1-MA to be a promising candidate to mimic and control the 3D biomechanical environment of cardiac cells. Competing Interests: H.A. van Boxtel is an employee of Fujifilm Manufacturing Europe B.V. The results of this study were not influenced by him or any other employee of Fujifilm Manufacturing Europe B.V. (© 2023 The Author(s).)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu