Prognostic value of midkine, syndecan-1, hyaluronan synthase-2, sestrin-1, laminin subunit alpha-4 and fibulin-3 for malignant pleural mesothelioma.

Autor: Akgun H; Eskisehir Osmangazi University Lung and Pleural Cancers Research and Clinical Center, Eskisehir, Turkey.; Department of Chest Diseases, Medical Faculty, Eskisehir Osmangazi University, Eskisehir, Turkey., Metintas S; Eskisehir Osmangazi University Lung and Pleural Cancers Research and Clinical Center, Eskisehir, Turkey.; Department of Public Health, Medical Faculty, Eskisehir Osmangazi University, Eskisehir, Turkey., Ak G; Eskisehir Osmangazi University Lung and Pleural Cancers Research and Clinical Center, Eskisehir, Turkey.; Department of Chest Diseases, Medical Faculty, Eskisehir Osmangazi University, Eskisehir, Turkey., Demirkol Canlı S; Department of Molecular Biology and Genetics, İhsan Doğramacı Bilkent University, Ankara, Turkey., Isbilen M; Department of Molecular Biology and Genetics, İhsan Doğramacı Bilkent University, Ankara, Turkey., Gure AO; Department of Molecular Biology and Genetics, İhsan Doğramacı Bilkent University, Ankara, Turkey., Metintas M; Eskisehir Osmangazi University Lung and Pleural Cancers Research and Clinical Center, Eskisehir, Turkey.; Department of Chest Diseases, Medical Faculty, Eskisehir Osmangazi University, Eskisehir, Turkey.
Jazyk: angličtina
Zdroj: Archives of medical science : AMS [Arch Med Sci] 2021 Mar 25; Vol. 19 (2), pp. 355-364. Date of Electronic Publication: 2021 Mar 25 (Print Publication: 2023).
DOI: 10.5114/aoms/112525
Abstrakt: Introduction: The prognosis of malignant pleural mesothelioma (MPM) is poor, with a limited survival time. In this study, we aimed to examine expression levels of genes selected from relevant literature and to utilize in silico methods to determine genes whose expression could reflect the prognosis of patients with MPM by ex-vivo validation experiments.
Material and Methods: The study group consisted of 54 MPM patients treated with chemotherapy. Expression of 6 genes - midkine (MDK), syndecan-1 (SDC1), hyaluronan synthase-2 (HAS2), sestrin-1 (SESN1), laminin subunit alpha-4 (LAMA4), and fibulin-3 (FBLN3) - was examined by qPCR in tumor tissues. Sestrin-1 and LAMA4 were identified using an in house R-based script: Unsupervised Survival Analysis Tool. Midkine, SDC1, HAS2, and FBLN3 were selected from current literature. We used two housekeeping genes, i.e. glucose-6-phosphate dehydrogenase and TATA-box binding protein, as controls.
Results: Of the patients, 43 (79.6%) had epithelioid mesothelioma. The median survival for all patients was 10 (±1.2 SE) months (95% CI: 7.7-12.3). In multivariate analyses, MDK ( p = 0.007), HAS2 ( p = 0.008) and SESN1 ( p = 0.014) expression levels were related to survival time in the whole group. In epithelioid type MPM patients, MDK ( p = 0.014), FBLN3 ( p = 0.029), HAS2 ( p = 0.014) and SESN1 ( p = 0.045) expression was related to survival time in multivariate analyses.
Conclusions: High HAS2 and SESN1 expressions and low MDK are potential biomarkers of good prognosis in MPM. High HAS2 and SESN1 expression and low MDK and FBLN3 can also be utilized as biomarkers of good prognosis for epithelioid MPM. Those results should be further investigated in sera, plasma, and pleural effusions.
Competing Interests: The authors declare no conflict of interest.
(Copyright: © 2021 Termedia & Banach.)
Databáze: MEDLINE
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