Telomerase RNA component lncRNA as potential diagnostic biomarker promotes CRC cellular migration and apoptosis evasion via modulation of β-catenin protein level.

Autor:	Bakr M; Department of Biochemistry, Faculty of Pharmacy, University of Sadat City, Sadat City, Egypt., Abd-Elmawla MA; Department of Biochemistry, Faculty of Pharmacy, Cairo University, Cairo, Egypt., Elimam H; Department of Biochemistry, Faculty of Pharmacy, University of Sadat City, Sadat City, Egypt.; Department of Biochemistry, Faculty of Pharmacy, Sinai University, Kantara, Egypt., Gamal El-Din H; Department of Surgical Oncology, National Cancer Institute, Cairo University, Cairo, Egypt., Fawzy A; Department of Clinical and Chemical Pathology, National Cancer Institute, Cairo University, Cairo, Egypt., Abulsoud AI; Department of Biochemistry, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo, Egypt.; Department of Biochemistry, Faculty of Pharmacy, Heliopolis University, Cairo, Egypt., Rizk SM; Department of Biochemistry, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
Jazyk:	angličtina
Zdroj:	Non-coding RNA research [Noncoding RNA Res] 2023 Mar 21; Vol. 8 (3), pp. 302-314. Date of Electronic Publication: 2023 Mar 21 (Print Publication: 2023).
DOI:	10.1016/j.ncrna.2023.03.004
Abstrakt:	Aim: Long non-coding RNA (LncRNA) telomerase RNA component (TERC) has telomerase-dependent and independent activity in numerous cancer types. The present study purposes to demonstrate the role of lncRNA TERC as a diagnostic serum biomarker in colorectal cancer (CRC) patients and the molecular mechanism of lncRNA TERC in inducing tumor in CRC cell lines. Materials and Methods: PCR array was performed to examine lncRNAs dysregulated in CRC. LncRNA TERC expression level was evaluated in 70 CRC patients and 35 control subjects using RT-qPCR. Then transfection was performed to build down-expression models of lncRNA TERC. ROC curve analysis was applied to assess the diagnostic value of serum LncRNA CRC. In addition, RT-qPCR was used to detect expression level of lncRNA TERC and β-catenin mRNA. Moreover, ELISA and Western blot were used to detect the level of β-catenin protein in sera of CRC patients and cell lines. The biological functions such as cell growth and migration of CRC cells were assessed using a wound healing assay. Cell cycle analysis and apoptosis analysis were performed using flow cytometry. Results: The lncRNA TERC is overexpressed in the sera of CRC patients with high diagnostic and stage discrimination accuracy. Furthermore, lncRNA TERC expression was upregulated in CRC cell lines and lncRNA TERC silencing induced cell arrest and apoptosis and inhibited cell migration. Furthermore, inhibition of lncRNA TERC reduces β-catenin protein levels. Conclusion: The lncRNA TERC could be considered as an early stages CRC diagnostic biomarker with a good ability to discriminate between CRC stages. lncRNA TERC induces CRC by promoting cell migration and evading apoptosis by elevating the level of β-catenin protein. Competing Interests: The authors declare that they have no conflict of interest. (© 2023 The Authors.)
Databáze:	MEDLINE
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