Musa spp. cultivars as a neutralising source against some toxic activities of Bothrops and Crotalus genus snake venoms.

Autor: Matos da Silva M; Laboratório de Produtos Naturais e Síntese Orgânica, Instituto de Química, Universidade Federal de Goiás, 74.690-900, Goiânia, GO, Brazil., Pereira Alexandre G; Laboratório de Produtos Naturais e Síntese Orgânica, Instituto de Química, Universidade Federal de Goiás, 74.690-900, Goiânia, GO, Brazil., Magalhães MR; Centro de Estudos e Pesquisas Biológicas, Escola de Ciências Médicas e da Vida, Pontifícia Universidade Católica de Goiás, 74.605-010, Goiânia, GO, Brazil., Torres AM; Laboratorio de Produtos Naturales Prof. Armando Ricciardi, Universidad Nacional del Nordeste, CP 3400, Corrientes, Argentina., Kato L; Laboratório de Produtos Naturais e Síntese Orgânica, Instituto de Química, Universidade Federal de Goiás, 74.690-900, Goiânia, GO, Brazil., Costa da Silva V; Laboratório de Comportamento Celular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, 74.690-900, Goiânia, GO, Brazil., Teixeira de Saboia Morais SM; Laboratório de Comportamento Celular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, 74.690-900, Goiânia, GO, Brazil., Garcia Rodriguez A; Laboratório de Bioquímica e Biotecnologia, Instituto de Ciências Biológicas, Universidade Federal de Goiás, 74.690-900, Goiânia, GO, Brazil., Pacheco Fill T; Laboratório Institucional de Espectrometria de Massas, Instituto de Química, Universidade Estadual de Campinas, 13.083-970, Campinas, SP, Brazil., Pereira AK; Laboratório Institucional de Espectrometria de Massas, Instituto de Química, Universidade Estadual de Campinas, 13.083-970, Campinas, SP, Brazil., Roque J; Laboratório de Cromatografia e Espectrometria de Massas, Instituto de Química, Universidade Federal de Goiás, 74.690-900, Goiânia, GO, Brazil., Souza Simão JL; Laboratório de Produtos Naturais e Síntese Orgânica, Instituto de Química, Universidade Federal de Goiás, 74.690-900, Goiânia, GO, Brazil., Pasqualotto Severino VG; Laboratório de Produtos Naturais e Síntese Orgânica, Instituto de Química, Universidade Federal de Goiás, 74.690-900, Goiânia, GO, Brazil. Electronic address: vanessapasqualotto@ufg.br.
Jazyk: angličtina
Zdroj: Toxicon : official journal of the International Society on Toxinology [Toxicon] 2023 Jun 01; Vol. 228, pp. 107106. Date of Electronic Publication: 2023 Apr 07.
DOI: 10.1016/j.toxicon.2023.107106
Abstrakt: Accidents involving snakes from Bothrops spp. and Crotalus spp. constitute the most important cause of envenomation in Brazil and Argentina. Musa spp. (banana) have been reported to be used in popular medicine against snakebite by the members of the Canudos Settlement, located in Goiás. In this way, the aim of this work was to evaluate the antivenom effect of the Ouro (AA), Prata (AAB), Prata-anã (AAB) and Figo (ABB) cultivars against in vitro (phospholipase, coagulation and proteolytic) and in vivo (lethality and toxicity) activities caused by the venoms and toxicity (Artemia salina nauplii and Danio rerio embryos) of Musa spp. as well as the annotation of chemical compounds possibly related to these activities. From the in vitro antiophidic tests with the sap, we observed 100% inhibition of the phospholipase and coagulant activities with the cultivars Prata-anã and Figo against the venoms of B. alternatus and C. d. collineatus, B. diporus and B. pauloensis, respectively, and neutralisation of the lethality against the B. diporus venom. It was observed that the cultivars of Musa spp. did not show toxicity against Artemia salina nauplii and Danio rerio embryos. The sap analysis via HPLC-MS/MS allowed the annotation of the 13 compounds: abscisic acid, shikimic acid, citric acid, quinic acid, afzelechin, Glp-hexose, glucose, sucrose, isorhamnetin-3-O-galactoside-6-raminoside, kaempferol-3-glucoside-3-raminoside, myricetin-3-O-rutinoside, procyanidin B1 and rutin. Therefore, it can be seen that Musa spp. is a potential therapeutic agent that can act to neutralise the effects caused by snakebites.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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