Methacrylated fibrinogen hydrogels for 3D cell culture and delivery.

Autor: Simaan-Yameen H; Faculty of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa 32000, Israel; Interdisciplinary Biotechnology Program, Technion - Israel Institute of Technology, Haifa 32000, Israel., Bar-Am O; Faculty of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa 32000, Israel., Saar G; Faculty of Medicine, Technion-Israel Institute of Technology, Haifa 32000, Israel., Seliktar D; Faculty of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa 32000, Israel. Electronic address: dror@bm.technion.ac.il.
Jazyk: angličtina
Zdroj: Acta biomaterialia [Acta Biomater] 2023 Jul 01; Vol. 164, pp. 94-110. Date of Electronic Publication: 2023 Apr 06.
DOI: 10.1016/j.actbio.2023.03.046
Abstrakt: Methacrylation was performed on fibrinogen to design a new biomedical hydrogel for 3D cell culture or as a biodegradable delivery matrix for in vivo implantation. The methacrylation of denatured fibrinogen in solution was performed using methacrylic anhydride (MAA). The extent of fibrinogen methacrylation was quantified by proton NMR and controlled using stochiometric quantities of MAA during the reaction. The methacrylated fibrinogen (FibMA) hydrogels were formed by light-activated free-radical polymerization in the presence of macromolecular cross-linking polymers made from acrylated poly(ethylene glycol) (PEG). The biocompatibility and biodegradability of the FibMA hydrogels were characterized by in vitro assays and in vivo implantation experiments using quantitative magnetic resonance imaging (MRI) of the implant volume. The FibMA supported the growth and metabolic activity of human dermal fibroblasts in both 2D and 3D cultures. The methacrylation did not alter important biological attributes of the fibrinogen, including the ability to support cell adhesion and 3D cell culture, as well as to undergo proteolysis. Animal experiments confirmed the biodegradability of the FibMA for potential use as a scaffold in tissue engineering, as a bioink for 3D bioprinting, or as a biodegradable matrix for in vivo sustained delivery of bioactive factors. STATEMENT OF SIGNIFICANCE: This paper describes methacrylated fibrinogen (FibMA) and the formation of a biomedical hydrogel from FibMA for cell culture and other biomedical applications. Inspired from methacrylated gelatin (GelMA), the FibMA is made from blood-derived fibrinogen which is more suitable for clinical use. Sharing similar properties to other hydrogels made from methacrylated proteins, the FibMA has yet to be reported in the literature. In this manuscript, we provide the methodology to produce the FibMA hydrogels, we document the mechanical versatility of this new biomaterial, and we show the biocompatibility using 3D cell culture studies and in vivo implantations.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023. Published by Elsevier Ltd.)
Databáze: MEDLINE
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