Mesenchymal stromal cell-derived membrane particles: A novel cell-free therapy for inflammatory bowel diseases.

Autor: Serafini MA; Cells, Tissue and Genes Laboratory, Experimental Research Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil; Biological Sciences - Physiology Graduate Program, Basic Health Sciences Institute, Federal University of Rio Grande do Sul, Porto Alegre, Brazil., Sirena DH; Cells, Tissue and Genes Laboratory, Experimental Research Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil; Biological Sciences - Physiology Graduate Program, Basic Health Sciences Institute, Federal University of Rio Grande do Sul, Porto Alegre, Brazil., da Silveira ABT; Cells, Tissue and Genes Laboratory, Experimental Research Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil., Franco-da-Silva M; Cells, Tissue and Genes Laboratory, Experimental Research Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil; Biological Sciences - Physiology Graduate Program, Basic Health Sciences Institute, Federal University of Rio Grande do Sul, Porto Alegre, Brazil., Aubin MR; Cells, Tissue and Genes Laboratory, Experimental Research Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil., Garcez TNA; Animal Experimentation Unit, Experimental Research Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil., Araújo A; Umbilical Cord Blood Bank, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil., Dos Santos Pereira F; Cells, Tissue and Genes Laboratory, Experimental Research Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil., Hoogduijn MJ; Erasmus MC Transplant Institute, Internal Medicine, Erasmus Medical Center, Rotterdam, Netherlands., da Costa Gonçalves F; Erasmus MC Transplant Institute, Internal Medicine, Erasmus Medical Center, Rotterdam, Netherlands., Paz AH; Cells, Tissue and Genes Laboratory, Experimental Research Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil; Biological Sciences - Physiology Graduate Program, Basic Health Sciences Institute, Federal University of Rio Grande do Sul, Porto Alegre, Brazil; Morphological Sciences Department, Basic Health Sciences Institute, Federal University of Rio Grande do Sul, Porto Alegre, Brazil. Electronic address: apaz@hcpa.edu.br.
Jazyk: angličtina
Zdroj: International immunopharmacology [Int Immunopharmacol] 2023 May; Vol. 118, pp. 110076. Date of Electronic Publication: 2023 Apr 06.
DOI: 10.1016/j.intimp.2023.110076
Abstrakt: Inflammatory bowel diseases (IBD), including ulcerative colitis, are chronic and idiopathic inflammations of the gastrointestinal tract. A disruption of the epithelial barrier and an imbalance between Th1 and Th2 subsets are associated with the onset and progression of these diseases. Mesenchymal stromal cells (MSC) are a promising therapy for IBD. However, cell-tracking studies have shown that intravenously infused MSC localize to the lungs and present short-term survival. To reduce practical complexities arising from living cells, we generated membrane particles (MP) from MSC membranes, which possess some of the immunomodulatory properties of MSC. This study investigated the effect of MSC-derived MP and conditioned media (CM) as cell-free therapies in the dextran sulfate sodium (DSS)-induced colitis model. Acute colitis was induced in C57BL/6 mice by oral administration of 2% DSS in drinking water ad libitum from days 0 to 7. Mice were treated with MP, CM, or living MSC on days 2 and 5. Our findings revealed that MP, CM, and living MSC ameliorated DSS-induced colitis by reducing colonic inflammation, the loss of colonic goblet cells, and intestinal mucosa permeability, preventing apoptosis of damaged colonic cells and balancing Th1 and Th2 activity. Therefore, MSC-derived MP have high therapeutic potential for treating IBD, overcoming the deficiencies of living MSC therapy, and opening novel frontiers in inflammatory diseases medicine.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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