AccsHSP21.7 enhances the antioxidant capacity of Apis cerana cerana.

Autor: Huang Y; State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, China., Zhang Y; State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, China., Niu X; State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, China., Sun Y; State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, China., Wang H; College of Animal Science and Technology, Shandong Agricultural University, Taian, China., Guo X; State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, China., Xu B; College of Animal Science and Technology, Shandong Agricultural University, Taian, China., Wang C; State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, China.
Jazyk: angličtina
Zdroj: Journal of the science of food and agriculture [J Sci Food Agric] 2023 Aug 30; Vol. 103 (11), pp. 5401-5411. Date of Electronic Publication: 2023 Apr 20.
DOI: 10.1002/jsfa.12614
Abstrakt: Background: The widespread use of glyphosate has many adverse effects on Apis cerana cerana. Due to the incomplete understanding of the molecular mechanisms of glyphosate toxicity, there are no available methods for mitigating the threat of glyphosate to Apis cerana cerana. Small heat shock proteins (sHSPs) play an important role in resisting oxidative stress, but their mechanism of action in Apis cerana cerana remains unclear.
Results: In this experiment, we cloned and identified AccsHSP21.7. Studies have shown that AccsHSP21.7 contains binding motifs for various transcription factors related to oxidative stress. Abiotic stresses induced the expression of AccsHSP21.7. Bacteriostatic testing of a recombinant AccsHSP21.7 protein proved that Escherichia coli overexpressing AccsHSP21.7 showed increased resistance to oxidative stress. Knocking down the AccsHSP21.7 gene caused significant damage to midgut cells, which seriously disrupted the antioxidant system in Apis cerana cerana and greatly increased mortality under glyphosate stress.
Conclusion: This study investigated the relationship between antioxidant regulation and the AccsHSP21.7 gene at the molecular level, and the results have guiding significance for the improvement of stress resistance in Apis cerana cerana. © 2023 Society of Chemical Industry.
(© 2023 Society of Chemical Industry.)
Databáze: MEDLINE
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