Injectable Colloidal Hydrogels of N -Vinylformamide Microgels Dispersed in Covalently Interlinked pH-Responsive Methacrylic Acid-Based Microgels.

Autor: Wang X; School of Materials, University of Manchester, MECD Building A, Manchester M1 7HL, U.K., Adlam DJ; Division of Cell Matrix Biology and Regenerative Medicine, The University of Manchester, Oxford Road, Manchester M13 9PT, U.K., Wang R; School of Materials, University of Manchester, MECD Building A, Manchester M1 7HL, U.K., Altujjar A; School of Materials, University of Manchester, MECD Building A, Manchester M1 7HL, U.K., Jia Z; School of Materials, University of Manchester, MECD Building A, Manchester M1 7HL, U.K., Saunders JM; School of Materials, University of Manchester, MECD Building A, Manchester M1 7HL, U.K., Hoyland JA; Division of Cell Matrix Biology and Regenerative Medicine, The University of Manchester, Oxford Road, Manchester M13 9PT, U.K., Rai N; School of Materials, University of Manchester, MECD Building A, Manchester M1 7HL, U.K., Saunders BR; School of Materials, University of Manchester, MECD Building A, Manchester M1 7HL, U.K.
Jazyk: angličtina
Zdroj: Biomacromolecules [Biomacromolecules] 2023 May 08; Vol. 24 (5), pp. 2173-2183. Date of Electronic Publication: 2023 Apr 07.
DOI: 10.1021/acs.biomac.3c00058
Abstrakt: Injectable hydrogels offer great potential to augment damaged or degenerated soft tissues. A key criterion for such gels is that their modulus is as close as possible to that of the target tissue. The majority of synthetic hydrogels have used low molecular weight polymer chains which may cause problems if they diffuse away from the injection site and/or increase the local osmotic pressure. We previously introduced a different approach of injecting preformed ultra-high molecular weight pH-responsive microgels (MGs) that interlink to form hydrogels. MGs are crosslinked polymer colloid particles that swell when the pH approaches the particle p K a . These colloidal hydrogels are termed doubly crosslinked microgels (DX MGs). The gel moduli of previous DX MGs were much greater than that reported for human nucleus pulposus (NP) tissue of the spinal intervertebral disk. Here, we replace some of the pH-responsive poly(ethyl acrylate- co -methacrylic acid) (PEA-MAA) MGs with hydrophilic non-ionic MGs based on poly( N -vinylformamide) (NVF). We investigate the morphology and mechanical properties of these new injectable composite DX MGs and show that the mechanical properties can be tuned by systematically varying the NVF MG content. Using this approach, the gel moduli close to that for NP tissue are achieved. These injectable new pH-responsive gels exhibit low cytotoxicity. Our work provides a potential new system for minimally invasive intervertebral disk augmentation.
Databáze: MEDLINE
Externí odkaz: