Exploring the microbiome of oral epithelial dysplasia as a predictor of malignant progression.

Autor: Wright RJ; Department of Pharmacology, Dalhousie University, Halifax, Canada. Robyn.Wright@dal.ca., Pewarchuk ME; Department of Integrative Oncology, British Columbia Cancer Research Centre, Vancouver, Canada., Marshall EA; Department of Integrative Oncology, British Columbia Cancer Research Centre, Vancouver, Canada., Murrary B; Department of Pharmacology, Dalhousie University, Halifax, Canada., Rosin MP; Department of Cancer Control Research, British Columbia Cancer Research Centre, Vancouver, Canada.; Department of Biomedical Physiology and Kinesiology, Simon Fraser University, Burnaby, Canada., Laronde DM; Department of Cancer Control Research, British Columbia Cancer Research Centre, Vancouver, Canada.; Faculty of Dentistry, University of British Columbia, Vancouver, Canada., Zhang L; Faculty of Dentistry, University of British Columbia, Vancouver, Canada.; Oral Biopsy Service, Vancouver General Hospital, Vancouver, Canada., Lam WL; Department of Integrative Oncology, British Columbia Cancer Research Centre, Vancouver, Canada., Langille MGI; Department of Pharmacology, Dalhousie University, Halifax, Canada.; Beatrice Hunter Cancer Research Institute, Halifax, Canada., Rock LD; Department of Pharmacology, Dalhousie University, Halifax, Canada.; Beatrice Hunter Cancer Research Institute, Halifax, Canada.; Faculty of Dentistry, Dalhousie University, Halifax, Canada.; Department of Pathology, Faculty of Medicine, Dalhousie University, Halifax, Canada.; Department of Anatomical Pathology, QEII Hospital, Nova Scotia Health, Halifax, Canada.
Jazyk: angličtina
Zdroj: BMC oral health [BMC Oral Health] 2023 Apr 06; Vol. 23 (1), pp. 206. Date of Electronic Publication: 2023 Apr 06.
DOI: 10.1186/s12903-023-02911-5
Abstrakt: A growing body of research associates the oral microbiome and oral cancer. Well-characterized clinical samples with outcome data are required to establish relevant associations between the microbiota and disease. The objective of this study was to characterize the community variations and the functional implications of the microbiome in low-grade oral epithelial dysplasia (OED) using 16S rRNA gene sequencing from annotated archival swabs in progressing (P) and non-progressing (NP) OED. We characterised the microbial community in 90 OED samples - 30 swabs from low-grade OED that progressed to cancer (cases) and 60 swabs from low-grade OED that did not progress after a minimum of 5 years of follow up (matched control subjects). There were small but significant differences between P and NP samples in terms of alpha diversity as well as beta diversity in conjunction with other clinical factors such as age and smoking status for both taxa and functional predictions. Across all samples, the most abundant genus was Streptococcus, followed by Haemophilus, Rothia, and Neisseria. Taxa and predicted functions were identified that were significantly differentially abundant with progression status (all Ps and NPs), when samples were grouped broadly by the number of years between sampling and progression or in specific time to progression for Ps only. However, these differentially abundant features were typically present only at low abundances. For example, Campylobacter was present in slightly higher abundance in Ps (1.72%) than NPs (1.41%) and this difference was significant when Ps were grouped by time to progression. Furthermore, several of the significantly differentially abundant functions were linked to the Campylobacteraceae family in Ps and may justify further investigation. Larger cohort studies to further explore the microbiome as a potential biomarker of risk in OED are warranted.
(© 2023. The Author(s).)
Databáze: MEDLINE
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