Analysis of the structure and function of the LYK cluster of Medicago truncatula A17 and R108.

Autor: Luu TB; Laboratory of Plant-Microbe-Environment Interactions (LIPME), Université de Toulouse, INRAE, CNRS, Castanet-Tolosan, France., Carles N; Laboratory of Plant-Microbe-Environment Interactions (LIPME), Université de Toulouse, INRAE, CNRS, Castanet-Tolosan, France., Bouzou L; Laboratory of Plant-Microbe-Environment Interactions (LIPME), Université de Toulouse, INRAE, CNRS, Castanet-Tolosan, France., Gibelin-Viala C; Laboratory of Plant-Microbe-Environment Interactions (LIPME), Université de Toulouse, INRAE, CNRS, Castanet-Tolosan, France., Remblière C; Laboratory of Plant-Microbe-Environment Interactions (LIPME), Université de Toulouse, INRAE, CNRS, Castanet-Tolosan, France., Gasciolli V; Laboratory of Plant-Microbe-Environment Interactions (LIPME), Université de Toulouse, INRAE, CNRS, Castanet-Tolosan, France., Bono JJ; Laboratory of Plant-Microbe-Environment Interactions (LIPME), Université de Toulouse, INRAE, CNRS, Castanet-Tolosan, France., Lefebvre B; Laboratory of Plant-Microbe-Environment Interactions (LIPME), Université de Toulouse, INRAE, CNRS, Castanet-Tolosan, France., Pauly N; Laboratory of Plant-Microbe-Environment Interactions (LIPME), Université de Toulouse, INRAE, CNRS, Castanet-Tolosan, France; Institut Sophia Agrobiotech, Université Côte d'Azur, INRAE, CNRS, Sophia Antipolis Cedex, France. Electronic address: nicolas.pauly@inrae.fr., Cullimore J; Laboratory of Plant-Microbe-Environment Interactions (LIPME), Université de Toulouse, INRAE, CNRS, Castanet-Tolosan, France.
Jazyk: angličtina
Zdroj: Plant science : an international journal of experimental plant biology [Plant Sci] 2023 Jul; Vol. 332, pp. 111696. Date of Electronic Publication: 2023 Apr 04.
DOI: 10.1016/j.plantsci.2023.111696
Abstrakt: The establishment of the Legume-Rhizobia symbiosis is generally dependent on the production of rhizobial lipochitooligosaccharidic Nod factors (NFs) and their perception by plant Lysin Motif Receptor-Like Kinases (LysM-RLKs). In this study, we characterized a cluster of LysM-RLK genes implicated in strain-specific recognition in two highly divergent and widely-studied Medicago truncatula genotypes, A17 and R108. We then used reverse genetic approaches and biochemical analyses to study the function of selected genes in the clusters and the ability of their encoded proteins to bind NFs. Our study has revealed that the LYK cluster exhibits a high degree of variability among M. truncatula genotypes, which in A17 and R108 includes recent recombination events within the cluster and a transposon insertion in A17. The essential role of LYK3 in nodulation in A17 is not conserved in R108 despite similar sequences and good nodulation expression profiles. Although, LYK2, LYK5 and LYK5bis are not essential for nodulation of the two genotypes, some evidence points to accessory roles in nodulation, but not through high-affinity NF binding. This work shows that recent evolution in the LYK cluster provides a source of variation for nodulation, and potential robustness of signaling through genetic redundancy.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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