RENEB Inter-Laboratory Comparison 2021: The Dicentric Chromosome Assay.

Autor:	Endesfelder D; Bundesamt für Strahlenschutz, BfS, Oberschleissheim, Germany., Oestreicher U; Bundesamt für Strahlenschutz, BfS, Oberschleissheim, Germany., Bucher M; Bundesamt für Strahlenschutz, BfS, Oberschleissheim, Germany., Beinke C; Bundeswehr Institute of Radiobiology, Munich, Germany., Siebenwirth C; Bundeswehr Institute of Radiobiology, Munich, Germany., Ainsbury E; UK Health Security Agency, Radiation, Chemicals and Environmental Hazards Directorate, Chilton, Oxfordshire, United Kingdom., Moquet J; UK Health Security Agency, Radiation, Chemicals and Environmental Hazards Directorate, Chilton, Oxfordshire, United Kingdom., Gruel G; Institut de Radioprotection et de Sûreté Nucléaire (IRSN), PSE-Santé, SERAMED, LRAcc Fontenay-aux-Roses 92262, France., Gregoire E; Institut de Radioprotection et de Sûreté Nucléaire (IRSN), PSE-Santé, SERAMED, LRAcc Fontenay-aux-Roses 92262, France., Martinez JS; Institut de Radioprotection et de Sûreté Nucléaire (IRSN), PSE-Santé, SERAMED, LRAcc Fontenay-aux-Roses 92262, France., Vral A; Faculty of Medicine and Health Sciences, Universiteit Gent, Gent, Belgium., Baeyens A; Faculty of Medicine and Health Sciences, Universiteit Gent, Gent, Belgium., Valente M; Armed Forces Biomedical Research Institute, Department of Radiation Biological, Effects Brétigny-sur-Orge, France., Montoro A; Laboratorio de Dosimetría Biológica Servicio de Protección Radiológica Hospital Universitario Politécnico la Fe, Spain., Terzoudi G; National Centre for Scientific Research 'Demokritos,' Health Physics, Radiobiology & Cytogenetics Laboratory, Athens, Greece., Triantopoulou S; National Centre for Scientific Research 'Demokritos,' Health Physics, Radiobiology & Cytogenetics Laboratory, Athens, Greece., Pantelias A; National Centre for Scientific Research 'Demokritos,' Health Physics, Radiobiology & Cytogenetics Laboratory, Athens, Greece., Monteiro Gil O; Centro de Ciências e Tecnologias Nucleares, Instituto Superior Técnico (IST), Universidade de Lisboa, Lisboa, Portugal., Prieto MJ; Hospital General Universitario Gregorio Marañón; Servicio de Oncología Radioterápica; Laboratorio de dosimetría biológica, Madrid, Spain., Domene MM; Hospital General Universitario Gregorio Marañón; Servicio de Oncología Radioterápica; Laboratorio de dosimetría biológica, Madrid, Spain., Zafiropoulos D; Laboratori Nazionali di Legnaro - Istituto Nazionale di Fisica Nucleare, Legnaro, Italy., Barquinero JF; Universitat Autònoma de Barcelona, Bellaterra, Spain., Pujol-Canadell M; Universitat Autònoma de Barcelona, Bellaterra, Spain., Lumniczky K; Radiation Medicine Unit, Department of Radiobiology and Radiohygiene, National Public Health Centre, Budapest, Hungary., Hargitai R; Radiation Medicine Unit, Department of Radiobiology and Radiohygiene, National Public Health Centre, Budapest, Hungary., Kis E; Radiation Medicine Unit, Department of Radiobiology and Radiohygiene, National Public Health Centre, Budapest, Hungary., Testa A; Agenzia nazionale per le nuove tecnologie, l'energia e lo sviluppo economico sostenibile, Rome, Italy., Patrono C; Agenzia nazionale per le nuove tecnologie, l'energia e lo sviluppo economico sostenibile, Rome, Italy., Sommer S; Institute of Nuclear Chemistry and Technology, Warsaw, Poland., Hristova R; National Centre of Radiobiology and Radiation Protection, Sofia, Bulgaria., Kostova N; National Centre of Radiobiology and Radiation Protection, Sofia, Bulgaria., Atanasova M; National Centre of Radiobiology and Radiation Protection, Sofia, Bulgaria., Sevriukova O; Radiation Protection Centre, Department of Expertise and Exposure Monitoring, Vilnius, Lithuania., Domínguez I; Universidad de Sevilla, Departamento de Biología Celular, Facultad de Biología, Sevilla, Spain., Pastor N; Universidad de Sevilla, Departamento de Biología Celular, Facultad de Biología, Sevilla, Spain., Güçlü I; Nükleer Arş Ens. Yarımburgaz mah. Nükleer Arş yolu, Turkey., Pajic J; Serbian Institute of Occupational Health, Belgrade, Serbia., Sabatier L; PROCyTOX, Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), Fontenay-aux-Roses, France and Université Paris-Saclay, France., Brochard P; PROCyTOX, Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), Fontenay-aux-Roses, France and Université Paris-Saclay, France., Tichy A; Department of Radiobiology, Faculty of Military Health Sciences, University of Defence, Hradec Králové, Czech Republic., Milanova M; Department of Radiobiology, Faculty of Military Health Sciences, University of Defence, Hradec Králové, Czech Republic., Finot F; Genevolution, Porcheville, France., Cuceu Petrenci C; Genevolution, Porcheville, France., Wilkins RC; Consumer and Clinical Radiation Protection Bureau, Health Canada, Ottawa, Canada., Beaton-Green LA; Consumer and Clinical Radiation Protection Bureau, Health Canada, Ottawa, Canada., Seong KM; Lab of Biological Dosimetry, National Radiation Emergency Medical Center, Korea Institute of Radiological & Medical Sciences, Seoul, Republic of Korea., Lee Y; Lab of Biological Dosimetry, National Radiation Emergency Medical Center, Korea Institute of Radiological & Medical Sciences, Seoul, Republic of Korea., Lee YH; Lab of Biological Dosimetry, National Radiation Emergency Medical Center, Korea Institute of Radiological & Medical Sciences, Seoul, Republic of Korea., Balajee AS; Cytogenetic Biodosimetry Laboratory; Radiation Emergency Assistance Center/Training Site (REAC/TS); Oak Ridge Institute for Science and Education; Oak Ridge Associated Universities; Oak Ridge, Tennessee., Maznyk N; Radiation Cytogenetics Laboratory; S.P. Grigoriev Institute for Medical Radiology and Oncology of Ukrainian National Academy of Medical Science, Kharkiv, Ukraine., Sypko T; Radiation Cytogenetics Laboratory; S.P. Grigoriev Institute for Medical Radiology and Oncology of Ukrainian National Academy of Medical Science, Kharkiv, Ukraine., Pham ND; Biodosimetry Laboratory, Center for Radiation Technology & Biotechnology; Dalat Nuclear Research Institute; Dalat City, Vietnam., Tran TM; Biodosimetry Laboratory, Center for Radiation Technology & Biotechnology; Dalat Nuclear Research Institute; Dalat City, Vietnam., Miura T; Department of Risk Analysis and Biodosimetry Institute of Radiation Emergency Medicine, Hirosaki University, Hirosaki, Japan., Suto Y; National Institutes for Quantum Science and Technology, Chiba, Japan., Akiyamam M; National Institutes for Quantum Science and Technology, Chiba, Japan., Tsuyama N; Department of Radiation Life Sciences, Fukushima Medical University School of Medicine, Fukushima, Japan., Abe Y; Department of Radiation Biology and Protection, Atomic Bomb Disease Institute, Nagasaki University, Japan., Goh VST; Department of Radiobiology, Singapore Nuclear Research and Safety Initiative (SNRSI), National University of Singapore, Singapore., Chua CEL; Department of Radiobiology, Singapore Nuclear Research and Safety Initiative (SNRSI), National University of Singapore, Singapore., Abend M; Bundeswehr Institute of Radiobiology, Munich, Germany., Port M; Bundeswehr Institute of Radiobiology, Munich, Germany.
Jazyk:	angličtina
Zdroj:	Radiation research [Radiat Res] 2023 Jun 01; Vol. 199 (6), pp. 556-570.
DOI:	10.1667/RADE-22-00202.1
Abstrakt:	After large-scale radiation accidents where many individuals are suspected to be exposed to ionizing radiation, biological and physical retrospective dosimetry assays are important tools to aid clinical decision making by categorizing individuals into unexposed/minimally, moderately or highly exposed groups. Quality-controlled inter-laboratory comparisons of simulated accident scenarios are regularly performed in the frame of the European legal association RENEB (Running the European Network of Biological and Physical retrospective Dosimetry) to optimize international networking and emergency readiness in case of large-scale radiation events. In total 33 laboratories from 22 countries around the world participated in the current RENEB inter-laboratory comparison 2021 for the dicentric chromosome assay. Blood was irradiated in vitro with X rays (240 kVp, 13 mA, ∼75 keV, 1 Gy/min) to simulate an acute, homogeneous whole-body exposure. Three blood samples (no. 1: 0 Gy, no. 2: 1.2 Gy, no. 3: 3.5 Gy) were sent to each participant and the task was to culture samples, to prepare slides and to assess radiation doses based on the observed dicentric yields from 50 manually or 150 semi-automatically scored metaphases (triage mode scoring). Approximately two-thirds of the participants applied calibration curves from irradiations with γ rays and about 1/3 from irradiations with X rays with varying energies. The categorization of the samples in clinically relevant groups corresponding to individuals that were unexposed/minimally (0-1 Gy), moderately (1-2 Gy) or highly exposed (>2 Gy) was successfully performed by all participants for sample no. 1 and no. 3 and by ≥74% for sample no. 2. However, while most participants estimated a dose of exactly 0 Gy for the sham-irradiated sample, the precise dose estimates of the samples irradiated with doses >0 Gy were systematically higher than the corresponding reference doses and showed a median deviation of 0.5 Gy (sample no. 2) and 0.95 Gy (sample no. 3) for manual scoring. By converting doses estimated based on γ-ray calibration curves to X-ray doses of a comparable mean photon energy as used in this exercise, the median deviation decreased to 0.27 Gy (sample no. 2) and 0.6 Gy (sample no. 3). The main aim of biological dosimetry in the case of a large-scale event is the categorization of individuals into clinically relevant groups, to aid clinical decision making. This task was successfully performed by all participants for the 0 Gy and 3.5 Gy samples and by 74% (manual scoring) and 80% (semiautomatic scoring) for the 1.2 Gy sample. Due to the accuracy of the dicentric chromosome assay and the high number of participating laboratories, a systematic shift of the dose estimates could be revealed. Differences in radiation quality (X ray vs. γ ray) between the test samples and the applied dose effect curves can partly explain the systematic shift. There might be several additional reasons for the observed bias (e.g., donor effects, transport, experimental conditions or the irradiation setup) and the analysis of these reasons provides great opportunities for future research. The participation of laboratories from countries around the world gave the opportunity to compare the results on an international level. (©2023 by Radiation Research Society. All rights of reproduction in any form reserved.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu