Staphylococcus nasal colonization in three species of non-human primates.

Autor: da Silva JG; Instituto de Tecnologia em Imunobiológicos-Bio-Manguinhos/Fiocruz, Avenida Brasil 4365, Pavilhão Rocha Lima, sala 412, Rio de Janeiro, RJ, CEP21040-900, Brazil., Culuchi G; Instituto de Tecnologia em Imunobiológicos-Bio-Manguinhos/Fiocruz, Avenida Brasil 4365, Pavilhão Rocha Lima, sala 412, Rio de Janeiro, RJ, CEP21040-900, Brazil., Pestana CP; Instituto de Tecnologia em Imunobiológicos-Bio-Manguinhos/Fiocruz, Avenida Brasil 4365, Pavilhão Rocha Lima, sala 412, Rio de Janeiro, RJ, CEP21040-900, Brazil., da Silva Junior HC; Instituto de Tecnologia em Imunobiológicos-Bio-Manguinhos/Fiocruz, Avenida Brasil 4365, Pavilhão Rocha Lima, sala 412, Rio de Janeiro, RJ, CEP21040-900, Brazil., Saraiva FB; Instituto de Tecnologia em Imunobiológicos-Bio-Manguinhos/Fiocruz, Avenida Brasil 4365, Pavilhão Rocha Lima, sala 412, Rio de Janeiro, RJ, CEP21040-900, Brazil., Kugelmeier T; Laboratório de Pesquisa em Infecção Hospitalar do Instituto Oswaldo Cruz (LAPIH/IOC/Fiocruz), Rio de Janeiro, Brazil., Rouede D; Laboratório de Pesquisa em Infecção Hospitalar do Instituto Oswaldo Cruz (LAPIH/IOC/Fiocruz), Rio de Janeiro, Brazil., Pinto ACA; Laboratório de Pesquisa em Infecção Hospitalar do Instituto Oswaldo Cruz (LAPIH/IOC/Fiocruz), Rio de Janeiro, Brazil., Pissinati T; Laboratório de Pesquisa em Infecção Hospitalar do Instituto Oswaldo Cruz (LAPIH/IOC/Fiocruz), Rio de Janeiro, Brazil., D'Alincourt Assef AP; Serviço de Criação de Primatas não Humanos do Instituto de Ciência e Tecnologia em Biomodelos (ICTB/Fiocruz), Rio de Janeiro, Brazil., Rocha-de-Souza CM; Serviço de Criação de Primatas não Humanos do Instituto de Ciência e Tecnologia em Biomodelos (ICTB/Fiocruz), Rio de Janeiro, Brazil., E Oliveira TRT; Serviço de Criação de Primatas não Humanos do Instituto de Ciência e Tecnologia em Biomodelos (ICTB/Fiocruz), Rio de Janeiro, Brazil., Senna JPM; Instituto de Tecnologia em Imunobiológicos-Bio-Manguinhos/Fiocruz, Avenida Brasil 4365, Pavilhão Rocha Lima, sala 412, Rio de Janeiro, RJ, CEP21040-900, Brazil. jprocopio@bio.fiocruz.br.
Jazyk: angličtina
Zdroj: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2023 Jun; Vol. 54 (2), pp. 1267-1274. Date of Electronic Publication: 2023 Apr 04.
DOI: 10.1007/s42770-023-00959-7
Abstrakt: Bacterial nasal colonization is common in many mammals and Staphylococcus represents the main pathogen isolated. Staphylococcus nasal carriage in humans constitutes a risk factor for Staphylococcus infections pointing out the need for animal experimentation for nasal colonization studies, especially for vaccine development. A limitation in addressing this hypothesis has been a lack of appropriate animal model. Murine models do not mimic human nasal colonization studies. Non-human primates (NHP) remain the best classical models for nasal colonization studies. In this study, we analyzed nasal colonization between two species of Old World monkeys (cynomolgus and rhesus) and a New World monkey (squirrel monkey) from breeding colony at Fiocruz (Brazil). Sixty male and female NHP with the average age of 1-21 years old, comprising twenty animals of each species, were analyzed. Nine different Staphylococcus species (S. aureus, S. cohnii, S. saprophyticus, S. haemolyticus, S. xylosus, S. warneri, S. nepalensis, S. simiae, and S. kloosi) were identified by MALDI-TOF and 16S rRNA gene sequence analyses. Antibiotic resistance was not detected among the isolated bacterial population. S. aureus was the main isolate (19 strains), present in all species, predominant in cynomolgus monkeys (9/20) and squirrel monkeys (7/20). spa typing was used to examine the clonal structure and genetic profile of Staphylococcus aureus isolates. Eight (8) spa types were identified among the S. aureus strains. A major cluster was identified, corresponding to a new spa type t20455, and no spa types found in this study were seen before in Brazil.
(© 2023. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.)
Databáze: MEDLINE
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