Noninvasive metabolic profiling of cumulus cells, oocytes, and embryos via fluorescence lifetime imaging microscopy: a mini-review.
| Autor: | Venturas M; Molecular and Cellular Biology and School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, USA.; Boston IVF-The Eugin Group, Waltham, MA, USA., Yang X; Molecular and Cellular Biology and School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, USA.; Cluster of Excellence Physics of Life, TU Dresden, Dresden, Germany., Sakkas D; Boston IVF-The Eugin Group, Waltham, MA, USA., Needleman D; Molecular and Cellular Biology and School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, USA.; Center for Computational Biology, Flatiron Institute, New York, NY, USA. |
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| Jazyk: | angličtina |
| Zdroj: | Human reproduction (Oxford, England) [Hum Reprod] 2023 May 02; Vol. 38 (5), pp. 799-810. |
| DOI: | 10.1093/humrep/dead063 |
| Abstrakt: | A major challenge in ART is to select high-quality oocytes and embryos. The metabolism of oocytes and embryos has long been linked to their viability, suggesting the potential utility of metabolic measurements to aid in selection. Here, we review recent work on noninvasive metabolic imaging of cumulus cells, oocytes, and embryos. We focus our discussion on fluorescence lifetime imaging microscopy (FLIM) of the autofluorescent coenzymes NAD(P)H and flavine adenine dinucleotide (FAD+), which play central roles in many metabolic pathways. FLIM measurements provide quantitative information on NAD(P)H and FAD+ concentrations and engagement with enzymes, leading to a robust means of characterizing the metabolic state of cells. We argue that FLIM is a promising approach to aid in oocyte and embryo selection. (© The Author(s) 2023. Published by Oxford University Press on behalf of European Society of Human Reproduction and Embryology. All rights reserved. For permissions, please email: journals.permissions@oup.com.) |
| Databáze: | MEDLINE |
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