Circular RNA circ-TNRC6B inhibits the proliferation and invasion of esophageal squamous cell carcinoma cells by regulating the miR-452-5p/DAG1 axis.

Autor: Xu R; Research Center, the Fourth Hospital of Hebei Medical University, Shijiazhuang, China., Ding P; The Third Department of Surgery, the Fourth Hospital of Hebei Medical University, Shijiazhuang, China.; Hebei Key Laboratory of Precision Diagnosis and Comprehensive Treatment of Gastric Cancer, the Fourth Hospital of Hebei Medical University, Shijiazhuang, China., Zhao X; Research Center, the Fourth Hospital of Hebei Medical University, Shijiazhuang, China., Li Z; Research Center, the Fourth Hospital of Hebei Medical University, Shijiazhuang, China., Liu F; Research Center, the Fourth Hospital of Hebei Medical University, Shijiazhuang, China., Gu L; Research Center, the Fourth Hospital of Hebei Medical University, Shijiazhuang, China., Zheng Y; Research Center, the Fourth Hospital of Hebei Medical University, Shijiazhuang, China.; Tumor Research Institute, the Fourth Hospital of Hebei Medical University, Shijiazhuang, China., Sang M; Research Center, the Fourth Hospital of Hebei Medical University, Shijiazhuang, China.; Tumor Research Institute, the Fourth Hospital of Hebei Medical University, Shijiazhuang, China., Meng L; Research Center, the Fourth Hospital of Hebei Medical University, Shijiazhuang, China.; Tumor Research Institute, the Fourth Hospital of Hebei Medical University, Shijiazhuang, China.
Jazyk: angličtina
Zdroj: Molecular oncology [Mol Oncol] 2023 Jul; Vol. 17 (7), pp. 1437-1452. Date of Electronic Publication: 2023 Apr 16.
DOI: 10.1002/1878-0261.13432
Abstrakt: Previous studies have uncovered the key role of circular RNAs (circRNAs) in various diseases, including cancer. However, the growth-inhibitory effects of circRNAs on esophageal squamous cell carcinoma (ESCC) have not been completely elucidated. This study characterized a newly identified circRNA derived from exons 9-13 of TNRC6B (named circ-TNRC6B). The expression of circ-TNRC6B in ESCC tissues was markedly downregulated when compared to that in non-tumor tissues. In 53 ESCC cases, circ-TNRC6B expression was negatively correlated with the T stage. Multivariate Cox regression analysis showed that circ-TNRC6B upregulation was an independent protective factor for ESCC patients' prognosis. Overexpression and knockdown functional experiments demonstrated that circ-TNRC6B inhibited ESCC cell proliferation, migration, and invasion. RNA immunoprecipitation and dual-luciferase reporter assays demonstrated that circ-TNRC6B sponges oncogenic miR-452-5p to upregulate the expression and activity of DAG1. Treatment with miR-452-5p inhibitor partially reversed the circ-TNRC6B-induced changes in the biological behavior of ESCC cells. These findings demonstrated that circ-TNRC6B exerts a tumor-suppressing effect in ESCC through the miR-452-5p/DAG1 axis. Thus, circ-TNRC6B is a potential prognostic biomarker for the clinical management of ESCC.
(© 2023 The Authors. Molecular Oncology published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.)
Databáze: MEDLINE
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