| Autor: |
Fernandes E Mendonça LM; Department of Pharmacology, Goa College of Pharmacy, Panaji, Goa, India., Joshi AB; Department of Pharmacognosy and Phytochemistry, Goa College of Pharmacy, Panaji, Goa, India., Bhandarkar A; Department of Pharmacognosy and Phytochemistry, Goa College of Pharmacy, Panaji, Goa, India., Joshi H; Department of Pharmacology, College of Pharmacy, Graphic Era Hill University, Bhimtal Campus, Bhimtal, Uttarakhand, India., Joshi S; Department of Pharmaceutical Chemistry, S.E.T.'s College of Pharmacy, Dharwad, Karnataka, India. |
| Jazyk: |
angličtina |
| Zdroj: |
Natural product research [Nat Prod Res] 2024 Feb-Mar; Vol. 38 (5), pp. 768-772. Date of Electronic Publication: 2023 Apr 04. |
| DOI: |
10.1080/14786419.2023.2197228 |
| Abstrakt: |
The study evaluated the therapeutic potential of ethanolic leaf extract of Piliostigma foveolatum (Dalzell) Thoth. (EEBF), its toluene, ethylacetate, methanol soluble fractions ( viz. TFBF, EFBF, MFBF), and isolated phytoconstituents against lung cancer. Four compounds were isolated from MFBF by column chromatography and preparative HPLC. Structures were elucidated by IR, 13 C-NMR, 1 H-NMR, mass spectroscopy and identified as Quercetin, Kaempferol, Isorhamnetin, and ß -glucogallin. EEBF and its biofractions exhibited remarkable antiproliferative activity with GI 50 <85µg/mL, while isolated Quercetin, Kaempferol, Isorhamnetin, and ß -Glucogallin displayed GI 50 values of 56.15 ± 1.16 μ M, 68.41 ± 3.98 μ M, 55.08 ± 0.57 μ M and 58.99 ± 12.39 μ M respectively. MFBF demonstrated significant apoptotic activity with 42.24 ± 0.57% cells in early and 4.61 ± 0.88% cells in late apoptosis comparable to standard Doxorubicin. Kaempferol exhibited 23.03 ± 0.37% cells in early and 2.11 ± 0.55% cells in late apoptosis, arresting Hop-62 cells in S-phase. In silico molecular docking, revealed that isolated constituents effectively bound to the same binding site of caspase-3 as Doxorubicin, highlighting their apoptotic mode of action. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
