QTL mapping of resistance to Pseudoperonospora cubensis clade 2, mating type A1, in Cucumis melo and dual-clade marker development.

Autor: Toporek SM; Department of Plant and Environmental Sciences, Clemson University, Coastal Research and Education Center, Charleston, SC, 29414, USA. stopore@clemson.edu., Branham SE; Department of Plant and Environmental Sciences, Clemson University, Coastal Research and Education Center, Charleston, SC, 29414, USA., Keinath AP; Department of Plant and Environmental Sciences, Clemson University, Coastal Research and Education Center, Charleston, SC, 29414, USA., Wechter WP; US Vegetable Laboratory, USDA, ARS, 2700 Savannah Highway, Charleston, SC, 29414, USA.
Jazyk: angličtina
Zdroj: TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik [Theor Appl Genet] 2023 Apr 03; Vol. 136 (4), pp. 91. Date of Electronic Publication: 2023 Apr 03.
DOI: 10.1007/s00122-023-04333-x
Abstrakt: Key Message: This is the first identification of QTLs underlying resistance in Cucumis melo to an isolate of Pseudoperonospora cubensis identified as Clade 2/mating type A1. Pseudoperonospora cubensis, causal organism of cucurbit downy mildew (CDM), causes severe necrosis and defoliation on Cucumis melo (melon). A recombinant inbred line population (N = 169) was screened against an isolate of P. cubensis (Clade 2/mating type A1) in replicated greenhouse and growth chamber experiments. SNPs (n = 5633 bins) identified in the RIL population were used for quantitative trait loci (QTL) mapping. A single major QTL on chromosome 10 (qPcub-10.3-10.4) was consistently associated with resistance across all experiments, while a second major QTL on chromosome 8 (qPcub-8.3) was identified only in greenhouse experiments. These two major QTLs were identified on the same chromosomes (8 and 10) but in different locations as two major QTLs (qPcub-8.2 and qPcub-10.1) previously identified for resistance to P. cubensis Clade 1/mating type A2. Kompetitive allele-specific PCR (KASP) markers were developed for these four major QTLs and validated in the RIL population through QTL mapping. These markers will provide melon breeders a high-throughput genotyping toolkit for development of melon cultivars with broad tolerance to CDM.
(© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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