Identification of potential bioactive compounds of Passiflora edulis leaf extract against colon adenocarcinoma cells.

Autor:	Villada Ramos JA; Facultad de Ciencias de la Salud, Universidad del Quindío, Armenia, Colombia.; Facultad de Ciencias Básicas y Tecnologías, Universidad del Quindío, Armenia, Colombia., Aguillón Osma J; Facultad de Ciencias de la Salud, Universidad del Quindío, Armenia, Colombia.; Facultad de Ciencias Básicas y Tecnologías, Universidad del Quindío, Armenia, Colombia., Restrepo Cortes B; Facultad de Ciencias de la Salud, Universidad del Quindío, Armenia, Colombia., Loango Chamarro N; Facultad de Ciencias de la Salud, Universidad del Quindío, Armenia, Colombia.; Facultad de Ciencias Básicas y Tecnologías, Universidad del Quindío, Armenia, Colombia., Maldonado Celis ME; Escuela de Nutrición y Dietética, Universidad de Antioquia, Apartado, 1226, Medellín, Colombia.
Jazyk:	angličtina
Zdroj:	Biochemistry and biophysics reports [Biochem Biophys Rep] 2023 Mar 28; Vol. 34, pp. 101453. Date of Electronic Publication: 2023 Mar 28 (Print Publication: 2023).
DOI:	10.1016/j.bbrep.2023.101453
Abstrakt:	Plant bioactive compounds such as flavonoids and triterpenes can affect lipid metabolism. Here, we report the cytotoxic and lipid-lowering activities of the ethanolic extract of P. edulis leaves on human colon adenocarcinoma SW480 cells, also the molecular interactions of bioactive compounds present in P. edulis extract on ACC and HMGCR enzymes. The extract reduced cell viability and decreased intracellular triglyceride content by up to 35% and 28% at 24 and 48 h, respectively; whereas the effect was evident on cholesterol only at 24 h. In-silico analysis revealed that luteolin, chlorogenic acid, moupinamide, isoorientin, glucosyl passionflower, cyclopasifloic acid E and saponarin had optimal molecular coupling on Acetyl-CoA Carboxylase 1 and 2 as well as 3-hydroxy-3-methyl-glutaryl-CoA reductase, with possible inhibitory effects. These results show the ability of ethanolic extract to reduce intracellular levels of cholesterol and triglycerides in SW480 cells, which attracts attention for the treatment of colorectal cancer. Competing Interests: The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Universidad del Quindio, 10.13039/501100005278Universidad de Antioquia reports financial support was provided by 10.13039/501100005278University of Antioquia. Maria Elena Maldonado Celis reports a relationship with University of Antioquia that includes: employment. The authors declare that they are not influenced by: 1. economic interests 2. personal interests 3. secondary benefits, and therefore it does not affect this research work. 4. None of the authors have editorial ties with this journal or the Elsevier group, 5. Nor any other benefit other than the dissemination of this new knowledge. (© 2023 The Authors.)
Databáze:	MEDLINE
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