Throughout in vitro first spermatogenic wave: Next-generation sequencing gene expression patterns of fresh and cryopreserved prepubertal mice testicular tissue explants.

Autor:	Dumont L; Univ Rouen Normandie, INSERM, NORDIC UMR 1239 - Team Adrenal and Gonadal Pathophysiology (AGoPath), Rouen, France.; Normandie, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France., Lopez Maestre H; Univ Rouen Normandie, INSERM, PANTHER UMR 1234, Rouen, France.; Institut Pasteur, Hub de Bioinformatique et Biostatistique - Département Biologie Computationnelle, USR 3756, CNRS, Paris, France., Chalmel F; University of Rennes 1, Inserm U1085-IRSET, Rennes, France., Huber L; Univ Rouen Normandie, INSERM, NORDIC UMR 1239 - Team Adrenal and Gonadal Pathophysiology (AGoPath), Rouen, France.; Normandie, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France., Rives-Feraille A; Univ Rouen Normandie, INSERM, NORDIC UMR 1239 - Team Adrenal and Gonadal Pathophysiology (AGoPath), Rouen, France.; Normandie, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France., Moutard L; Univ Rouen Normandie, INSERM, NORDIC UMR 1239 - Team Adrenal and Gonadal Pathophysiology (AGoPath), Rouen, France.; Normandie, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France., Bateux F; Univ Rouen Normandie, INSERM, NORDIC UMR 1239 - Team Adrenal and Gonadal Pathophysiology (AGoPath), Rouen, France.; Normandie, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France., Rondanino C; Univ Rouen Normandie, INSERM, NORDIC UMR 1239 - Team Adrenal and Gonadal Pathophysiology (AGoPath), Rouen, France.; Normandie, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France., Rives N; Univ Rouen Normandie, INSERM, NORDIC UMR 1239 - Team Adrenal and Gonadal Pathophysiology (AGoPath), Rouen, France.; Normandie, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France.; Rouen University Hospital, Biology of Reproduction-CECOS laboratory, Rouen, France.
Jazyk:	angličtina
Zdroj:	Frontiers in endocrinology [Front Endocrinol (Lausanne)] 2023 Mar 17; Vol. 14, pp. 1112834. Date of Electronic Publication: 2023 Mar 17 (Print Publication: 2023).
DOI:	10.3389/fendo.2023.1112834
Abstrakt:	Introduction: Suitable cryopreservation procedures of pre-pubertal testicular tissue associated with efficient culture conditions are crucial in the fields of fertility preservation and restoration. In vitro spermatogenesis remains a challenging technical procedure to undergo a complete spermatogenesis.The number of haploid cells and more specifically the spermatic yield produced in vitro in mice is still extremely low compared to age-matched in vivo controls and this procedure has never yet been successfully transferred to humans. Methods: To evaluate the impact of in vitro culture and freezing procedure, pre-pubertal testicular mice testes were directly cultured until day 4 (D4), D16 and D30 or cryopreserved by controlled slow freezing then cultured until D30. Testes composed of a panel of 6.5 dpp (days postpartum), 10.5 dpp, 22.5 dpp, and 36.5 dpp mice were used as in vivo controls. Testicular tissues were assessed by histological (HES) and immunofluorescence (stimulated by retinoic acid gene 8, STRA8) analyses. Moreover, a detailed transcriptome evaluation study has been carried out to study the gene expression patterns throughout the first in vitro spermatogenic wave. Results: Transcriptomic analyses reveal that cultured tissues expression profiles are almost comparable between D16 and D30; highlighting an abnormal kinetic throughout the second half of the first spermatogenesis during in vitro cultures. In addition, testicular explants have shown dysregulation of their transcriptomic profile compared to controls with genes related to inflammation response, insulin-like growth factor and genes involved in steroidogenesis. Discussion: The present work first shows that cryopreservation had very little impact on gene expression in testicular tissue, either directly after thawing or after 30 days in culture. Transcriptomic analysis of testis tissue samples is highly informative due to the large number of expressed genes and identified isoforms. This study provides a very valuable basis for future studies concerning in vitro spermatogenesis in mice. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Dumont, Lopez Maestre, Chalmel, Huber, Rives-Feraille, Moutard, Bateux, Rondanino and Rives.)
Databáze:	MEDLINE
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