Hemolysis scavenger proteins and renal function marker in children with sickle cell disease at steady state: A cross-sectional study.

Autor: Fordjour FA; Department of Microbiology University for Development Studies Tamale Ghana.; Department of Biochemistry and Biotechnology, College of Science Kwame Nkrumah University of Science and Technology Kumasi Ghana., Kwarteng A; Department of Biochemistry and Biotechnology, College of Science Kwame Nkrumah University of Science and Technology Kumasi Ghana.; Kumasi Centre for Collaborative Research in Tropical Medicine Kumasi Ghana., Paintsil V; Child Health Directorate Komfo Anokye Teaching Hospital Kumasi Ghana.; College of Health Sciences Kwame Nkrumah University of Science and Technology Kumasi Ghana., Amanor E; Department of Biochemistry and Biotechnology, College of Science Kwame Nkrumah University of Science and Technology Kumasi Ghana., Ackah EB; School of Public Health Kwame Nkrumah University of Science and Technology Kumasi Ghana., Amuzu EX; Child Health Directorate Komfo Anokye Teaching Hospital Kumasi Ghana., Sackey DS; Haematology Unit Komfo Anokye Teaching Hospital Kumasi Ghana., Akoto AOY; Child Health Directorate Komfo Anokye Teaching Hospital Kumasi Ghana.
Jazyk: angličtina
Zdroj: Health science reports [Health Sci Rep] 2023 Mar 30; Vol. 6 (4), pp. e1177. Date of Electronic Publication: 2023 Mar 30 (Print Publication: 2023).
DOI: 10.1002/hsr2.1177
Abstrakt: Background and Aims: Hemolysis is a fundamental feature of sickle cell disease (SCD) contributing to the vaso-occlusive crisis of patients. The objectives of the study were to assess the link between hemolysis proteins and hematological parameters, and to validate cystatin C (CYS C) as a potent renal marker in diagnoising SCD.
Method: Here, a cross-sectional study carried out at the pediatric SCD clinic of the Komfo Anokye Teaching Hospital comprised 90 SCD children (HbSC, HbSF, and HbSS). ANOVA, t -test, and Spearman's rank correlation analysis were done. Elevated proteins levels were compared to standard values; alpha-1 microglobulin (A1M) (1.8-65 µg/L), CYS C (0.1-4.5 µmol/L), and haemopexin (HPX) (500-1500 µg/mL).
Results: The mean (standard deviation) age of participants was 9.830 (±0.3217) years, and 46% of them were males. From simple descriptive analysis, we observed that all but one patient had their HPX level below the reference range (<500 µg/mL). Here, A1M levels were shown to be within the appropriate reference range for all the patients except few patients. CYS C levels were also all within the required reference values. A Spearman's rank correlation test between full blood count and HPX generally suggested a weak but positive correlation; RBC (coef. = 0.2448;  p  = 0.0248), HGB (coef. = 0.2310;  p  = 0.030), hematocrit (coef. = 0.2509; p  = 0.020), and platelet (coef. = 0.1545;  p  = 0.160). Mean corpuscular volume (coef. = -0.5645;  p  = 0.610) had a stronger but negative correlation with HPX. This study depicts a positive and stronger association between CYS C and HPX levels (coef. = 0.9996;  p  < 0.0001), validating the use of CYS C as a useful marker of renal function in persons with SCDs.
Conclusion: In the present study, we show that A1M levels were normal for most of the patients, hence CYS C levels are not alarming in this study. Further, there exists a correlation between hemolysis scavenger proteins and hematological parameters.
Competing Interests: The authors declare no conflict of interest.
(© 2023 The Authors. Health Science Reports published by Wiley Periodicals LLC.)
Databáze: MEDLINE
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