Impact of chronic alcohol exposure on conventional and regulatory murine T cell subsets.

Autor: Paterson CW; Department of Surgery, Emory University School of Medicine, Atlanta, GA, United States.; Medical Corps, United States Navy, Navy Reserve Officer Training Corps (NROTC), Atlanta, GA, United States.; Emory Critical Care Center, Emory University School of Medicine, Atlanta, GA, United States., Gutierrez MB; Department of Surgery, Emory University School of Medicine, Atlanta, GA, United States.; Emory Critical Care Center, Emory University School of Medicine, Atlanta, GA, United States., Coopersmith CM; Department of Surgery, Emory University School of Medicine, Atlanta, GA, United States., Ford ML; Department of Surgery, Emory University School of Medicine, Atlanta, GA, United States.; Emory Transplant Center, Emory University School of Medicine, Atlanta, GA, United States.
Jazyk: angličtina
Zdroj: Frontiers in immunology [Front Immunol] 2023 Mar 17; Vol. 14, pp. 1142614. Date of Electronic Publication: 2023 Mar 17 (Print Publication: 2023).
DOI: 10.3389/fimmu.2023.1142614
Abstrakt: Introduction: Chronic alcohol use poses significant negative consequences to public health and, among its many biologic effects, is associated with significant T cell dysregulation within the adaptive immune system that has yet to be fully characterized. Novel, automated strategies for high dimensional flow cytometric analysis of the immune system are rapidly improving researchers' ability to detect and characterize rare cell types.
Methods: Using a murine model of chronic alcohol ingestion in conjunction with viSNE and CITRUS analysis tools, we performed a machine-driven, exploratory analysis comparing rare splenic subpopulations within the conventional CD4 + , regulatory CD4 + and CD8 + T cell compartments between alcohol- and water-fed animals.
Results: While there were no differences in the absolute numbers of bulk CD3 + T cells, bulk CD4 + T cells, bulk CD8 + T cells, Foxp3 - CD4 + conventional T cells (T conv ) or Foxp3 + CD4 + regulatory T cells (T reg ), we identified populations of naïve Helios + CD4 + T conv and naïve CD103 + CD8 + splenic T cells that were decreased in chronically alcohol exposed mice versus water-fed controls. In addition, we identified increased CD69 + Treg and decreased CD103 + effector regulatory T cell (eT reg ) subsets in conjunction with increased frequency of a population that may represent a transitional phenotype between central regulatory T cell (cT reg ) and eT reg .
Discussion: These data provide further resolution into the character of decreased naïve T cell populations known to be present in alcohol exposed mice, as well as describe alterations in effector regulatory T cell phenotypes associated with the pathogenesis of chronic alcohol-induced immune dysfunction.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2023 Paterson, Gutierrez, Coopersmith and Ford.)
Databáze: MEDLINE
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