Influence of Biological and Environmental Factors in the Extraction and Concentration of Foodborne Pathogens using Glycan-Coated Magnetic Nanoparticles.

Autor:	Boodoo C; Nano-Biosensors Lab, Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA; Global Alliance for Rapid Diagnostics, Michigan State University, East Lansing, MI 48824, USA. Electronic address: boodooch@msu.edu., Dester E; Nano-Biosensors Lab, Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA; Global Alliance for Rapid Diagnostics, Michigan State University, East Lansing, MI 48824, USA. Electronic address: desterem@msu.edu., Asadullah Sharief S; Nano-Biosensors Lab, Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA; Global Alliance for Rapid Diagnostics, Michigan State University, East Lansing, MI 48824, USA. Electronic address: shariefs@msu.edu., Alocilja EC; Nano-Biosensors Lab, Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA; Global Alliance for Rapid Diagnostics, Michigan State University, East Lansing, MI 48824, USA. Electronic address: alocilja@msu.edu.
Jazyk:	angličtina
Zdroj:	Journal of food protection [J Food Prot] 2023 Apr; Vol. 86 (4), pp. 100066. Date of Electronic Publication: 2023 Feb 20.
DOI:	10.1016/j.jfp.2023.100066
Abstrakt:	Rapid detection of foodborne pathogens is essential to preventing foodborne illness outbreaks. Before detection can occur, however, it is often necessary to extract and concentrate bacteria. Conventional methods such as centrifugation, filtration, and immunomagnetic separation can often be time-consuming, ineffective, or costly when working with complex food matrices. This work used cost-effective glycan-coated magnetic nanoparticles (MNPs) for rapid concentration of Escherichia coli O157, Listeria monocytogenes, and Staphylococcus aureus. Glycan-coated MNPs were used to concentrate bacteria from both buffer solution and food matrices while examining the effect of factors including solution pH, bacterial concentration, and target bacterial species. In both pH 7 and reduced pH experiments, successful extraction of bacterial cells occurred in all food matrices and bacteria tested. In neutral pH buffer solution, bacteria were concentrated to 4.55 ± 1.17, 31.68 ± 6.10 and 64.27 ± 16.78 times their initial concentration (mean ± standard deviation) for E. coli, L. monocytogenes and S. aureus, respectively. Successful bacterial concentration occurred in several food matrices, including S. aureus in milk (pH 6), L. monocytogenes in sausage (pH 7), and E. coli O157 in flour (pH 7). The insights gained may facilitate future applications of glycan-coated MNPs to extract foodborne pathogens. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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