Diet prevents the expansion of segmented filamentous bacteria and ileo-colonic inflammation in a model of Crohn's disease.

Autor: Metwaly A; Chair of Nutrition and Immunology, Technical University of Munich, Freising, Germany., Jovic J; Chair of Nutrition and Immunology, Technical University of Munich, Freising, Germany., Waldschmitt N; Chair of Nutrition and Immunology, Technical University of Munich, Freising, Germany., Khaloian S; Chair of Nutrition and Immunology, Technical University of Munich, Freising, Germany., Heimes H; Chair of Nutrition and Immunology, Technical University of Munich, Freising, Germany., Häcker D; Chair of Nutrition and Immunology, Technical University of Munich, Freising, Germany., Ahmed M; Chair of Nutrition and Immunology, Technical University of Munich, Freising, Germany., Hammoudi N; APHP, Hôpital Saint Louis, Department of Gastroenterology, INSERM UMRS 1160, Paris Diderot, Sorbonne Paris-Cité University, Paris, France.; Université Paris Cité, INSERM U1160, EMiLy, Institut de Recherche Saint-Louis, Paris, France., Le Bourhis L; Université Paris Cité, INSERM U1160, EMiLy, Institut de Recherche Saint-Louis, Paris, France., Mayorgas A; Department of Experimental Pathology, Instituto de Investigaciones Biomédicas de Barcelona CSIC, IDIBAPS, CIBERehd, Barcelona, Spain., Siebert K; Department of Pediatrics, Dr. von Hauner Children's Hospital, University Hospital, LMU Munich, Munich, Germany., Basic M; Hannover Medical School, Institute for Laboratory Animal Science, Hannover, Germany., Schwerd T; Department of Pediatrics, Dr. von Hauner Children's Hospital, University Hospital, LMU Munich, Munich, Germany., Allez M; APHP, Hôpital Saint Louis, Department of Gastroenterology, INSERM UMRS 1160, Paris Diderot, Sorbonne Paris-Cité University, Paris, France.; Université Paris Cité, INSERM U1160, EMiLy, Institut de Recherche Saint-Louis, Paris, France., Panes J; Department of Experimental Pathology, Instituto de Investigaciones Biomédicas de Barcelona CSIC, IDIBAPS, CIBERehd, Barcelona, Spain., Salas A; Department of Experimental Pathology, Instituto de Investigaciones Biomédicas de Barcelona CSIC, IDIBAPS, CIBERehd, Barcelona, Spain., Bleich A; Hannover Medical School, Institute for Laboratory Animal Science, Hannover, Germany., Zeissig S; Department of Medicine I, University Hospital Dresden, Technische Universität (TU) Dresden, Dresden, Germany., Schnupf P; Université Paris Cité, INSERM UMR-S1151, CNRS UMR-S8253, Institut Necker Enfants Malades, F-75015, Paris, France., Cominelli F; Digestive Health Research Institute, Case Western Reserve University School of Medicine, Cleveland, OH, USA., Haller D; Chair of Nutrition and Immunology, Technical University of Munich, Freising, Germany. dirk.haller@tum.de.; ZIEL-Institute for Food and Health, Technical University of Munich, Freising, Germany. dirk.haller@tum.de.
Jazyk: angličtina
Zdroj: Microbiome [Microbiome] 2023 Mar 31; Vol. 11 (1), pp. 66. Date of Electronic Publication: 2023 Mar 31.
DOI: 10.1186/s40168-023-01508-y
Abstrakt: Background: Crohn's disease (CD) is associated with changes in the microbiota, and murine models of CD-like ileo-colonic inflammation depend on the presence of microbial triggers. Increased abundance of unknown Clostridiales and the microscopic detection of filamentous structures close to the epithelium of Tnf ΔARE mice, a mouse model of CD-like ileitis pointed towards segmented filamentous bacteria (SFB), a commensal mucosal adherent bacterium involved in ileal inflammation.
Results: We show that the abundance of SFB strongly correlates with the severity of CD-like ileal inflammation in two mouse models of ileal inflammation, including Tnf ΔARE and SAMP/Yit mice. SFB mono-colonization of germ-free Tnf ΔARE mice confirmed the causal link and resulted in severe ileo-colonic inflammation, characterized by elevated tissue levels of Tnf and Il-17A, neutrophil infiltration and loss of Paneth and goblet cell function. Co-colonization of SFB in human-microbiota associated Tnf ΔARE mice confirmed that SFB presence is indispensable for disease development. Screening of 468 ileal and colonic mucosal biopsies from adult and pediatric IBD patients, using previously published and newly designed human SFB-specific primer sets, showed no presence of SFB in human tissue samples, suggesting a species-specific functionality of the pathobiont. Simulating the human relevant therapeutic effect of exclusive enteral nutrition (EEN), EEN-like purified diet antagonized SFB colonization and prevented disease development in Tnf ΔARE mice, providing functional evidence for the protective mechanism of diet in modulating microbiota-dependent inflammation in IBD.
Conclusions: We identified a novel pathogenic role of SFB in driving severe CD-like ileo-colonic inflammation characterized by loss of Paneth and goblet cell functions in Tnf ΔARE mice. A purified diet antagonized SFB colonization and prevented disease development in Tnf ΔARE mice in contrast to a fiber-containing chow diet, clearly demonstrating the important role of diet in modulating a novel IBD-relevant pathobiont and supporting a direct link between diet and microbial communities in mediating protective functions. Video Abstract.
(© 2023. The Author(s).)
Databáze: MEDLINE
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