Hemocup blood feeder: An affordable and simplified blood-feeding device for maintenance of Aedes aegypti mosquito colonies in Sri Lanka.

Autor: Dilani PVD; Department of Chemistry, Faculty of Science, University of Colombo, Colombo, Sri Lanka. Electronic address: desha@chem.cmb.ac.lk., Wickramasinghe PDSU; Department of Chemistry, Faculty of Science, University of Colombo, Colombo, Sri Lanka. Electronic address: sachith@chem.cmb.ac.lk., Lakshman GVCP; Department of Chemistry, Faculty of Science, University of Colombo, Colombo, Sri Lanka. Electronic address: gvcpriyanath94@gmail.com., Ranathunge T; Department of Zoology, Faculty of Science, Eastern University of Sri Lanka, Vantharumoolai, Chenkalady, Sri Lanka. Electronic address: tharaka.ranathunge@gmail.com., Dassanayake RS; Department of Chemistry, Faculty of Science, University of Colombo, Colombo, Sri Lanka., Silva Gunawardene YIN; Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka. Electronic address: nilminisg@kln.ac.lk.
Jazyk: angličtina
Zdroj: Experimental parasitology [Exp Parasitol] 2023 Jun; Vol. 249, pp. 108518. Date of Electronic Publication: 2023 Mar 31.
DOI: 10.1016/j.exppara.2023.108518
Abstrakt: Dengue is a mosquito-borne viral disease mainly transmitted by Aedes aegypti and disease control is primarily reliant on mosquito vector control strategies. In the failure of conventional vector control strategies, new strategies are being developed which specifically require the maintenance of mosquito colonies in the laboratories. Blood-feeding is an essential part of the routine colony maintenance of Ae. aegypti. Therefore, the current study was focused on developing a simplified artificial membrane-feeding device, "Hemocup" feeder out of affordable material. viz., plastic cups, styrofoam insulation system, parafilm-M, and preheated water to facilitate the Ae. aegypti artificial blood feeding. The performance of the device was compared to that of a commercially available blood-feeding device, "Hemotek", by assessing the blood-feeding rate, fecundity, and egg hatchability. Similar blood feeding rates were observed for Hemocup and Hemotek methods (91.8 ± 1.6 and 94.3 ± 1.6 respectively>0.05) as well as comparable fecundity between the two methods (20.8 ± 0.7 and 22.0 ± 1.5 respectively; p > 0.05). Furthermore, there was no statistically significant difference in egg hatchability between the two methods (91.9 ± 1.4 and 93.8 ± 1.4, respectively; p > 0.05). The results indicate that this simple Hemocup blood-feeding system can be used for routine colonization of laboratory strains of Ae. aegypti and for mass-rearing purposes.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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