Protocol for a prospective cohort study exploring the gut microbiota of infants with congenital heart disease undergoing cardiopulmonary bypass (the GuMiBear study).

Autor:	Magner C; School of Nursing, Midwifery and Health Systems, University College Dublin, Dublin, Ireland., Jenkins D; Laboratory, Children's Health Ireland at Crumlin, Crumlin, Ireland., Koc F; School of Microbiology, University College Cork, Cork, Ireland.; Food Biosciences, Teagasc Food Research Centre, Cork, Ireland., Tan MH; Department Paediatric Cardiology, Children's Health Ireland at Crumlin, Dublin, Ireland., O'Toole M; Department Paediatric Cardiology, Children's Health Ireland at Crumlin, Dublin, Ireland., Boyle J; Department Paediatric Cardiology, Children's Health Ireland at Crumlin, Dublin, Ireland., Maguire N; Department Paediatric Cardiology, Children's Health Ireland at Crumlin, Dublin, Ireland., Duignan S; Department Paediatric Cardiology, Children's Health Ireland at Crumlin, Dublin, Ireland., Murphy K; University College Cork APC Microbiome Institute, Cork, Ireland.; Food Biosciences, Teagasc Food Research Centre, Moorepark, Ireland., Ross P; University College Cork College of Science Engineering and Food Science, Cork, Ireland., Stanton C; University College Cork APC Microbiome Institute, Cork, Ireland.; Teagasc Food Research Centre, Moorepark, Ireland., McMahon CJ; Department Paediatric Cardiology, Children's Health Ireland at Crumlin, Dublin, Ireland cmcmahon992004@yahoo.com.; University College Dublin School of Medicine, Dublin, Ireland.
Jazyk:	angličtina
Zdroj:	BMJ open [BMJ Open] 2023 Mar 31; Vol. 13 (3), pp. e067016. Date of Electronic Publication: 2023 Mar 31.
DOI:	10.1136/bmjopen-2022-067016
Abstrakt:	Introduction: The gut microbiota develops from birth and matures significantly during the first 24 months of life, playing a major role in infant health and development. The composition of the gut microbiota is influenced by several factors including mode of delivery, gestational age, feed type and treatment with antibiotics. Alterations in the pattern of gut microbiota development and composition can be associated with illness and compromised health outcomes.Infants diagnosed with 'congenital heart disease' (CHD) often require surgery involving cardiopulmonary bypass (CPB) early in life. The impact of this type of surgery on the integrity of the gut microbiome is poorly understood. In addition, these infants are at significant risk of developing the potentially devastating intestinal condition necrotising enterocolitis. Methods and Analysis: This study will employ a prospective cohort study methodology to investigate the gut microbiota and urine metabolome of infants with CHD undergoing surgery involving CPB. Stool and urine samples, demographic and clinical data will be collected from eligible infants based at the National Centre for Paediatric Cardiac Surgery in Ireland. Shotgun metagenome sequencing will be performed on stool samples and urine metabolomic analysis will identify metabolic biomarkers. The impact of the underlying diagnosis, surgery involving CPB, and the influence of environmental factors will be explored. Data from healthy age-matched infants from the INFANTMET study will serve as a control for this study. Ethics and Dissemination: This study has received full ethical approval from the Clinical Research Ethics Committee of Children's Health Ireland, GEN/826/20. Competing Interests: Competing interests: None declared. (© Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.)
Databáze:	MEDLINE
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