First molecular evidence of Wolbachia occurrence in Amblyomma sculptum (Acari: Ixodidae).

Autor: Romano DMM; Laboratory of Animal Parasitology, Animal Health Research Center, Biological Institute, São Paulo, SP, Brazil. Electronic address: dmmromano@gmail.com., Pereira TN; Vector Mosquito Group: Endosymbionts and Pathogen-Vector Interactions, René Rachou Institute, Fiocruz, Belo Horizonte, MG, Brazil., Almeida IB; Laboratory of Animal Parasitology, Animal Health Research Center, Biological Institute, São Paulo, SP, Brazil., Coelho CSG; Laboratory of Animal Parasitology, Animal Health Research Center, Biological Institute, São Paulo, SP, Brazil., Duarte FC; Laboratory of Animal Parasitology, Animal Health Research Center, Biological Institute, São Paulo, SP, Brazil., Harakava R; Reference Laboratory Unit in Molecular Biology, Biological Institute, São Paulo, SP, Brazil., Cassiano LL; Laboratory of Animal Parasitology, Animal Health Research Center, Biological Institute, São Paulo, SP, Brazil., Mendes MC; Laboratory of Animal Parasitology, Animal Health Research Center, Biological Institute, São Paulo, SP, Brazil.
Jazyk: angličtina
Zdroj: Veterinary parasitology [Vet Parasitol] 2023 May; Vol. 317, pp. 109907. Date of Electronic Publication: 2023 Mar 16.
DOI: 10.1016/j.vetpar.2023.109907
Abstrakt: As the main vector for the bacterium Rickettsia rickettsii in Brazil, the tick Amblyomma sculptum is a parasite of great public health importance in this country. Wolbachia is an endosymbiont bacterium highly widespread among invertebrates and because of its impact on its hosts' biology, form a powerful alternative for pests and disease control. The aim of this study was to investigate the occurrence of this bacterium in A. sculptum. For this, 187 adult ticks collected in two municipalities in the interior of the state of São Paulo, Brazil, were analyzed using molecular techniques and bioinformatics tools. A total of 15 ticks were positive for the presence of Wolbachia. Phylogenetic analysis on the 16S rRNA gene indicated that the Wolbachia DNA sequences obtained in this investigation belonged to different clades, probably in supergroups B and F. This was the first study to report the occurrence of Wolbachia in A. sculptum and it enriches knowledge about the susceptibility of ticks to this bacterium. Now that we know that Wolbachia can be found in A. sculptum, the objective for a next study must be to investigate Wolbachia's possible origin in this tick.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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