| Autor: |
McGrath M; Tissue Engineering Research Group, Department of Anatomy & Regenerative Medicine, Royal College of Surgeons in Ireland (RCSI), 123 St. Stephen's Green, Dublin D02 YN77, Ireland.; Advanced Materials and BioEngineering Research (AMBER) Centre, RCSI and TCD, Dublin D02 PN40, Ireland., Zimkowska K; Tissue Engineering Research Group, Department of Anatomy & Regenerative Medicine, Royal College of Surgeons in Ireland (RCSI), 123 St. Stephen's Green, Dublin D02 YN77, Ireland.; Regenerative Medicine Institute, University of Galway, Galway H91 TK33, Ireland., Genoud KJ; Tissue Engineering Research Group, Department of Anatomy & Regenerative Medicine, Royal College of Surgeons in Ireland (RCSI), 123 St. Stephen's Green, Dublin D02 YN77, Ireland.; Advanced Materials and BioEngineering Research (AMBER) Centre, RCSI and TCD, Dublin D02 PN40, Ireland.; Trinity Centre for Biomedical Engineering, Trinity College Dublin, Dublin 2 D02 PN40, Ireland., Maughan J; Tissue Engineering Research Group, Department of Anatomy & Regenerative Medicine, Royal College of Surgeons in Ireland (RCSI), 123 St. Stephen's Green, Dublin D02 YN77, Ireland.; Advanced Materials and BioEngineering Research (AMBER) Centre, RCSI and TCD, Dublin D02 PN40, Ireland.; School of Physics, Trinity College Dublin, Dublin D02 PN40, Ireland.; Centre for Research on Adaptive Nanostructures and Nanodevices (CRANN), Trinity College Dublin, Dublin 2 D02 W085, Ireland., Gutierrez Gonzalez J; Tissue Engineering Research Group, Department of Anatomy & Regenerative Medicine, Royal College of Surgeons in Ireland (RCSI), 123 St. Stephen's Green, Dublin D02 YN77, Ireland.; Advanced Materials and BioEngineering Research (AMBER) Centre, RCSI and TCD, Dublin D02 PN40, Ireland.; School of Chemistry, University of Dublin, Trinity College Dublin, Dublin 2 D02 W085, Ireland., Browne S; Tissue Engineering Research Group, Department of Anatomy & Regenerative Medicine, Royal College of Surgeons in Ireland (RCSI), 123 St. Stephen's Green, Dublin D02 YN77, Ireland., O'Brien FJ; Tissue Engineering Research Group, Department of Anatomy & Regenerative Medicine, Royal College of Surgeons in Ireland (RCSI), 123 St. Stephen's Green, Dublin D02 YN77, Ireland.; Advanced Materials and BioEngineering Research (AMBER) Centre, RCSI and TCD, Dublin D02 PN40, Ireland.; Trinity Centre for Biomedical Engineering, Trinity College Dublin, Dublin 2 D02 PN40, Ireland. |
| Abstrakt: |
Chronic, nonhealing wounds in the form of diabetic foot ulcers (DFUs) are a major complication for diabetic patients. The inability of a DFU to heal appropriately leads to an open wound with a high risk of infection. Current standards of care fail to fully address either the underlying defective wound repair mechanism or the risk of microbial infection. Thus, it is clear that novel approaches are needed. One such approach is the use of multifunctional biomaterials as platforms to direct and promote wound healing. In this study, a biomimetic, bilayered antimicrobial collagen-based scaffold was developed to deal with the etiology of DFUs. An epidermal, antimicrobial collagen/chitosan film for the prevention of wound infection was combined with a dermal collagen-glycosaminoglycan scaffold, which serves to support angiogenesis in the wound environment and ultimately accelerate wound healing. Biophysical and biological characterization identified an 1-ethyl-3-(3-(dimethylamino)propyl)carbodiimide cross-linked bilayered scaffold to have the highest structural stability with similar mechanical properties to products on the market, exhibiting a similar structure to native skin, successfully inhibiting the growth and infiltration of Staphylococcus aureus and supporting the proliferation of epidermal cells on its surface. This bilayered scaffold also demonstrated the ability to support the proliferation of key cell types involved in vascularization, namely, induced pluripotent stem cell derived endothelial cells and supporting stromal cells, with early signs of organization of these cells into vascular structures, showing great promise for the promotion of angiogenesis. Taken together, the results indicate that the bilayered scaffold is an excellent candidate for enhancement of diabetic wound healing by preventing wound infection and supporting angiogenesis. |
