Estimation of Two Diuretics Using Fluorescent Nitrogen Doped Carbon Quantum Dots: Application to Spiked Human Plasma and Tablets.

Autor: Abo Zaid MH; Pharmaceutical Chemistry Department, Faculty of Pharmacy, Delta University for Science and Technology, Gamasa, 35712, Egypt. mona.abozaid@deltauniv.edu.eg.; Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia, 41522, Egypt. mona.abozaid@deltauniv.edu.eg., El-Enany N; Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt.; Pharmaceutical Chemistry Department, Faculty of Pharmacy, New Mansoura University, New Mansoura, 7723730, Egypt., Mostafa AE; Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia, 41522, Egypt., Hadad GM; Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia, 41522, Egypt., Belal F; Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt.
Jazyk: angličtina
Zdroj: Journal of fluorescence [J Fluoresc] 2023 Nov; Vol. 33 (6), pp. 2209-2218. Date of Electronic Publication: 2023 Mar 31.
DOI: 10.1007/s10895-023-03217-z
Abstrakt: Highly fluorescent nitrogen doped carbon quantum dots (N-CQDs) were prepared by a single-step method based on microwave heating of cane sugar and urea. The produced N-CQDs were applied as nano-sensors for the spectrofluorimetric determination of eplerenone and spironolactone. A strong emission band at 376 nm was obtained after excitation at 216 nm due to the produced N-CQDs. The native fluorescence of N-CQDs was obviously quenched upon adding increased concentrations of each drug. A strong correlation was found between the fluorescence quenching of N-CQDs and the concentration of each drug. The method was found to be linear over the range of 0.5 to 5.0 μg/mL for eplerenone and 0.5 to 6.0 μg/mL for spironolactone with LOQ of 0.383 μg/mL and 0.262 μg/mL. The developed method was further extended for determination of both drugs in their pharmaceutical tablets and spiked human plasma. The results obtained were statistically compared with those of reported methods. The mechanism of fluorescence quenching of N-CQDs by the two drugs was discussed.
(© 2023. The Author(s).)
Databáze: MEDLINE
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