Virome-wide detection of natural infection events and the associated antibody dynamics using longitudinal highly-multiplexed serology.

Autor: Kelley EJ; The Translational Genomics Research Institute (TGen), Flagstaff and Phoenix, AZ, USA., Henson SN; The Translational Genomics Research Institute (TGen), Flagstaff and Phoenix, AZ, USA., Rahee F; The Translational Genomics Research Institute (TGen), Flagstaff and Phoenix, AZ, USA., Boyle AS; The Translational Genomics Research Institute (TGen), Flagstaff and Phoenix, AZ, USA., Engelbrektson AL; The Translational Genomics Research Institute (TGen), Flagstaff and Phoenix, AZ, USA., Nelson GA; The Translational Genomics Research Institute (TGen), Flagstaff and Phoenix, AZ, USA., Mead HL; The Translational Genomics Research Institute (TGen), Flagstaff and Phoenix, AZ, USA., Anderson NL; SISCAPA Assay Technologies, Inc., Washington, DC, USA., Razavi M; SISCAPA Assay Technologies, Inc., Washington, DC, USA., Yip R; SISCAPA Assay Technologies, Inc., Washington, DC, USA., Ladner JT; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ, USA., Scriba TJ; South African Tuberculosis Vaccine Initiative and Institute of Infectious Disease and Molecular Medicine, Division of Immunology, Department of Pathology, University of Cape Town, Cape Town, South Africa., Altin JA; The Translational Genomics Research Institute (TGen), Flagstaff and Phoenix, AZ, USA. jaltin@tgen.org.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2023 Mar 30; Vol. 14 (1), pp. 1783. Date of Electronic Publication: 2023 Mar 30.
DOI: 10.1038/s41467-023-37378-z
Abstrakt: Current methods for detecting infections either require a sample collected from an actively infected site, are limited in the number of agents they can query, and/or yield no information on the immune response. Here we present an approach that uses temporally coordinated changes in highly-multiplexed antibody measurements from longitudinal blood samples to monitor infection events at sub-species resolution across the human virome. In a longitudinally-sampled cohort of South African adolescents representing >100 person-years, we identify >650 events across 48 virus species and observe strong epidemic effects, including high-incidence waves of Aichivirus A and the D68 subtype of Enterovirus D earlier than their widespread circulation was appreciated. In separate cohorts of adults who were sampled at higher frequency using self-collected dried blood spots, we show that such events temporally correlate with symptoms and transient inflammatory biomarker elevations, and observe the responding antibodies to persist for periods ranging from ≤1 week to >5 years. Our approach generates a rich view of viral/host dynamics, supporting novel studies in immunology and epidemiology.
(© 2023. The Author(s).)
Databáze: MEDLINE
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