Bionanomining of copper-based nanoparticles using pre-processed mine tailings as the precursor.

Autor: das Neves Vasconcellos Brandão IY; Universidade Federal de São Paulo. Instituto de Ciência e Tecnologia; Departamento de Ciência e Tecnologia, São José dos Campos, SP, Brazil., Ferreira de Macedo E; Universidade Federal de São Paulo. Instituto de Ciência e Tecnologia; Departamento de Ciência e Tecnologia, São José dos Campos, SP, Brazil., Barboza de Souza Silva PH; Universidade Federal de São Paulo. Instituto de Ciência e Tecnologia; Departamento de Ciência e Tecnologia, São José dos Campos, SP, Brazil., Fontana Batista A; Instituto de Aeronáutica e Espaço (IAE), Departamento de Ciência e Tecnologia Aeroespacial (DCTA), Zip-code 12228-904, São José dos Campos, SP, Brazil., Graciano Petroni SL; Instituto de Aeronáutica e Espaço (IAE), Departamento de Ciência e Tecnologia Aeroespacial (DCTA), Zip-code 12228-904, São José dos Campos, SP, Brazil., Gonçalves M; Universidade Federal de São Paulo. Instituto de Ciência e Tecnologia; Departamento de Ciência e Tecnologia, São José dos Campos, SP, Brazil., Conceição K; Universidade Federal de São Paulo. Instituto de Ciência e Tecnologia; Departamento de Ciência e Tecnologia, São José dos Campos, SP, Brazil., de Sousa Trichês E; Universidade Federal de São Paulo. Instituto de Ciência e Tecnologia; Departamento de Ciência e Tecnologia, São José dos Campos, SP, Brazil., Batista Tada D; Universidade Federal de São Paulo. Instituto de Ciência e Tecnologia; Departamento de Ciência e Tecnologia, São José dos Campos, SP, Brazil., Maass D; Universidade Federal de São Paulo. Instituto de Ciência e Tecnologia; Departamento de Ciência e Tecnologia, São José dos Campos, SP, Brazil. Electronic address: danielle.maass@unifesp.br.
Jazyk: angličtina
Zdroj: Journal of environmental management [J Environ Manage] 2023 Jul 15; Vol. 338, pp. 117804. Date of Electronic Publication: 2023 Mar 28.
DOI: 10.1016/j.jenvman.2023.117804
Abstrakt: The bacterial synthesis of copper nanoparticles emerges as an eco-friendly alternative to conventional techniques since it comprises a single-step and bottom-up approach, which leads to stable metal nanoparticles. In this paper, we studied the biosynthesis of Cu-based nanoparticles by Rhodococcus erythropolis ATCC4277 using a pre-processed mining tailing as a precursor. The influence of pulp density and stirring rate on particle size was evaluated using a factor-at-time experimental design. The experiments were carried out in a stirred tank bioreactor for 24 h at 25 °C, wherein 5% (v/v) of bacterial inoculum was employed. The O 2 flow rate was maintained at 1.0 L min -1 and the pH at 7.0. Copper nanoparticles (CuNPs), with an average hydrodynamic diameter of 21 ± 1 nm, were synthesized using 25 g.L -1 of mining tailing and a stirring rate of 250 rpm. Aiming to visualize some possible biomedical applications of the as-synthesized CuNPs, their antibacterial activity was evaluated against Escherichia coli and their cytotoxicity was evaluated against Murine Embryonic Fibroblast (MEF) cells. The 7-day extract of CuNPs at 0.1 mg mL -1 resulted in 75% of MEF cell viability. In the direct method, the suspension of CuNPs at 0.1 mg mL -1 resulted in 70% of MEF cell viability. Moreover, the CuNPs at 0.1 mg mL -1 inhibited 60% of E. coli growth. Furthermore, the NPs were evaluated regarding their photocatalytic activity by monitoring the oxidation of methylene blue (MB) dye. The CuNPs synthesized showed rapid oxidation of MB dye, with the degradation of approximately 65% of dye content in 4 h. These results show that the biosynthesis of CuNPs by R. erythropolis using pre-processed mine tailing can be a suitable method to obtain CuNPs from environmental and economical perspectives, resulting in NPs useful for biomedical and photocatalytic applications.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Danielle Maass reports financial support was provided by The São Paulo Research Foundation - FAPESP.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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