| Autor: |
Hegde S; Departments of Vector Biology and Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool L3 5QA, UK., Brettell LE; Departments of Vector Biology and Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool L3 5QA, UK., Quek S; Departments of Vector Biology and Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool L3 5QA, UK., Etebari K; Australian Infectious Disease Research Centre, School of Biological Sciences, The University of Queensland, Brisbane, Queensland, Australia., Saldaña MA; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas, USA., Asgari S; Australian Infectious Disease Research Centre, School of Biological Sciences, The University of Queensland, Brisbane, Queensland, Australia., Coon KL; Department of Bacteriology, University of Wisconsin-Madison, Madison, WI, USA., Heinz E; Departments of Vector Biology and Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool L3 5QA, UK., Hughes GL; Departments of Vector Biology and Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool L3 5QA, UK. |
| Abstrakt: |
The mosquito microbiome is critical for host development and plays a major role in many aspects of mosquito biology. While the microbiome is commonly dominated by a small number of genera, there is considerable variation in composition among mosquito species, life stages, and geography. How the host controls and is affected by this variation is unclear. Using microbiome transplant experiments, we asked whether there were differences in transcriptional responses when mosquitoes of different species were used as microbiome donors. We used microbiomes from four different donor species spanning the phylogenetic breadth of the Culicidae, collected either from the laboratory or field. We found that when recipients received a microbiome from a donor reared in the laboratory, the response was remarkably similar regardless of donor species. However, when the donor had been collected from the field, far more genes were differentially expressed. We also found that while the transplant procedure did have some effect on the host transcriptome, this is likely to have had a limited effect on mosquito fitness. Overall, our results highlight the possibility that variation in mosquito microbiome communities are associated with variability in host-microbiome interactions and further demonstrate the utility of the microbiome transplantation technique. |
