Unravelling specific diet and gut microbial contributions to inflammatory bowel disease.

Autor: Pereira GV; Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan, USA., Boudaud M; Department of Infection and Immunity, Luxembourg Institute of Health, 4354 Esch-sur-Alzette, Luxembourg., Wolter M; Department of Infection and Immunity, Luxembourg Institute of Health, 4354 Esch-sur-Alzette, Luxembourg.; Faculty of Science, Technology and Medicine, University of Luxembourg, 4365 Esch-sur-Alzette, Luxembourg., Alexander C; Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan, USA., De Sciscio A; Department of Infection and Immunity, Luxembourg Institute of Health, 4354 Esch-sur-Alzette, Luxembourg., Grant ET; Department of Infection and Immunity, Luxembourg Institute of Health, 4354 Esch-sur-Alzette, Luxembourg.; Faculty of Science, Technology and Medicine, University of Luxembourg, 4365 Esch-sur-Alzette, Luxembourg., Trindade BC; Dept. of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA., Pudlo NA; Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan, USA., Singh S; Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan, USA., Campbell A; Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan, USA., Shan M; Dept. of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA.; Dept. of Molecular & Integrative Physiology, University of Michigan, Ann Arbor, Michigan, USA., Zhang L; Dept. of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA.; Dept. of Molecular & Integrative Physiology, University of Michigan, Ann Arbor, Michigan, USA., Willieme S; Department of Infection and Immunity, Luxembourg Institute of Health, 4354 Esch-sur-Alzette, Luxembourg., Kim K; Dept. of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA., Denike-Duval T; Unit for Laboratory Animal Medicine, University of Michigan, Ann Arbor, Michigan, USA., Bleich A; Institute for Laboratory Animal Science, Hanover Medical School, Hanover, Germany., Schmidt TM; Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan, USA.; Dept. of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA.; Dept. of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, Michigan, USA., Kennedy L; Unit for Laboratory Animal Medicine, University of Michigan, Ann Arbor, Michigan, USA., Lyssiotis CA; Dept. of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA.; Dept. of Molecular & Integrative Physiology, University of Michigan, Ann Arbor, Michigan, USA., Chen GY; Dept. of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA., Eaton KA; Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan, USA., Desai MS; Department of Infection and Immunity, Luxembourg Institute of Health, 4354 Esch-sur-Alzette, Luxembourg.; Odense Research Center for Anaphylaxis, Department of Dermatology and Allergy Center, Odense University Hospital, University of Southern Denmark, 5000 Odense, Denmark., Martens EC; Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan, USA.
Jazyk: angličtina
Zdroj: Research square [Res Sq] 2023 Mar 13. Date of Electronic Publication: 2023 Mar 13.
DOI: 10.21203/rs.3.rs-2518251/v1
Abstrakt: Inflammatory bowel disease (IBD) is a chronic condition characterized by periods of spontaneous intestinal inflammation and is increasing in industrialized populations. Combined with host genetic predisposition, diet and gut bacteria are thought to be prominent features contributing to IBD, but little is known about the precise mechanisms involved. Here, we show that low dietary fiber promotes bacterial erosion of protective colonic mucus, leading to lethal colitis in mice lacking the IBD-associated cytokine, interleukin-10. Diet-induced inflammation is driven by mucin-degrading bacteria-mediated Th1 immune responses and is preceded by expansion of natural killer T cells and reduced immunoglobulin A coating of some bacteria. Surprisingly, an exclusive enteral nutrition diet, also lacking dietary fiber, reduced disease by increasing bacterial production of isobutyrate, which is dependent on the presence of a specific bacterial species, Eubacterium rectale . Our results illuminate a mechanistic framework using gnotobiotic mice to unravel the complex web of diet, host and microbial factors that influence IBD.
Databáze: MEDLINE