Zika Virus Infection Damages the Testes in Pubertal Common Squirrel Monkeys ( Saimiri collinsi ).

Autor: Benchimol GDC; Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua 67030-000, Pará, Brazil.; Postgraduate Program in Virology (PPGV), Evandro Chagas Institute, Ananindeua 67030-000, Pará, Brazil., Santos JB; Laboratory of Amazon Animal Biotechnology and Medicine (BIOMEDAM), Federal University of Pará, Castanhal 68740-970, Pará, Brazil.; Postgraduate Program in Animal Reproduction in the Amazon (ReproAmazon), Federal University of Pará, Castanhal 68740-970, Pará, Brazil., Lopes ASDC; National Primate Center, Ananindeua 67000-000, Pará, Brazil., Oliveira KG; National Primate Center, Ananindeua 67000-000, Pará, Brazil., Okada EST; National Primate Center, Ananindeua 67000-000, Pará, Brazil., de Alcantara BN; Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua 67030-000, Pará, Brazil., Pereira WLA; Laboratory of Animal Pathology (LABOPAT), Institute of Health and Animal Production, Federal Rural University of the Amazon, Belém 66077-830, Pará, Brazil., Leão DL; Laboratory of Amazon Animal Biotechnology and Medicine (BIOMEDAM), Federal University of Pará, Castanhal 68740-970, Pará, Brazil.; Mamirauá Institute for Sustainable Development, Tefé 69553-225, Amazonas, Brazil., Martins ACC; National Primate Center, Ananindeua 67000-000, Pará, Brazil., Carneiro LA; National Primate Center, Ananindeua 67000-000, Pará, Brazil., Imbeloni AA; National Primate Center, Ananindeua 67000-000, Pará, Brazil., Makiama ST; National Primate Center, Ananindeua 67000-000, Pará, Brazil., de Castro LPPA; The Brazilian Institute for the Environment and Renewable Natural Resources, Belém 66087-441, Pará, Brazil., Coutinho LN; Laboratory of Animal Pathology (LABOPAT), Institute of Health and Animal Production, Federal Rural University of the Amazon, Belém 66077-830, Pará, Brazil., Casseb LMN; Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua 67030-000, Pará, Brazil.; Postgraduate Program in Virology (PPGV), Evandro Chagas Institute, Ananindeua 67030-000, Pará, Brazil., Vasconcelos PFDC; Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua 67030-000, Pará, Brazil.; Department of Pathology, Center of Biologic and Health Sciences, State University of Pará, Belém 66050-540, Pará, Brazil., Domingues SFS; Laboratory of Amazon Animal Biotechnology and Medicine (BIOMEDAM), Federal University of Pará, Castanhal 68740-970, Pará, Brazil.; Postgraduate Program in Animal Reproduction in the Amazon (ReproAmazon), Federal University of Pará, Castanhal 68740-970, Pará, Brazil.; School of Veterinary Medicine, Federal University of Pará, Castanhal 68740-970, Pará, Brazil., Medeiros DBA; Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua 67030-000, Pará, Brazil.; Postgraduate Program in Virology (PPGV), Evandro Chagas Institute, Ananindeua 67030-000, Pará, Brazil., Scalercio SRRA; National Primate Center, Ananindeua 67000-000, Pará, Brazil.
Jazyk: angličtina
Zdroj: Viruses [Viruses] 2023 Feb 23; Vol. 15 (3). Date of Electronic Publication: 2023 Feb 23.
DOI: 10.3390/v15030615
Abstrakt: During the Zika virus (ZIKV) outbreak and after evidence of its sexual transmission was obtained, concerns arose about the impact of the adverse effects of ZIKV infection on human fertility. In this study, we evaluated the clinical-laboratory aspects and testicular histopathological patterns of pubertal squirrel monkeys ( Saimiri collinsi ) infected with ZIKV, analyzing the effects at different stages of infection. The susceptibility of S. collinsi to ZIKV infection was confirmed by laboratory tests, which detected viremia (mean 1.63 × 10 6 RNA copies/µL) and IgM antibody induction. Reduced fecal testosterone levels, severe testicular atrophy and prolonged orchitis were observed throughout the experiment by ultrasound. At 21 dpi, testicular damage associated with ZIKV was confirmed by histopathological and immunohistochemical (IHC) analyses. Tubular retraction, the degeneration and necrosis of somatic and germ cells in the seminiferous tubules, the proliferation of interstitial cells and an inflammatory infiltrate were observed. ZIKV antigen was identified in the same cells where tissue injuries were observed. In conclusion, squirrel monkeys were found to be susceptible to the Asian variant of ZIKV, and this model enabled the identification of multifocal lesions in the seminiferous tubules of the infected group evaluated. These findings may suggest an impact of ZIKV infection on male fertility.
Databáze: MEDLINE
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